AG-WUS-PcG-lncRNA互作对梅多雌蕊发育的调控

项目名称： AG-WUS-PcG-lncRNA互作对梅多雌蕊发育的调控

项目编号： No.31500571

项目类型： 青年科学基金项目

立项/批准年度： 2016

项目学科： 农业科学

项目作者： 侍婷

作者单位： 南京农业大学

项目金额： 20万元

中文摘要： 梅完全花中通常含有一枚正常发育的雌蕊，但是有少数品种，例如‘品字梅’等，通常含有三至数枚雌蕊，其中部分能够在授粉后正常发育，形成一花多果的表型，可以大大提高果梅观赏价值及产量。本项目拟以一花一雌蕊品种‘青佳2号’和一花多雌蕊品种‘品字梅’为研究对象，通过物候期观察‘品字梅’多雌蕊现象，采用石蜡切片等解剖学研究来确定多雌蕊分化过程的关键时期；利用原位杂交技术观察 AGAMOUS（AG）、WUSCHEL（WUS）在花芽中的表达情况；检测AG及H3K27me3蛋白表达量情况，随后进行染色体免疫共沉淀反应（Chromatin Immunoprecipitation，ChIP），鉴定AG及H3K27me3蛋白与WUS的结合情况同时筛选两个品种间的差异表达的与PcG相关的long noncoding RNA（lncRNA）以提出AG-WUS-PcG-lncRNA在梅多雌蕊分化过程中的调

中文关键词： 雌蕊发育；离生雌蕊；转录因子；组蛋白修饰；非编码RNA调控

英文摘要： The perfect flower usually contains one normal pistil in Mei, but novel species, such as the ‘Pinzimei’, usually contain more than two pistils, and then some develop normally after pollination and form a phenotype, one flower owns two to four fruits, which can greatly improve the ornamental value and yield in Mei. We are planning to take the monocarpous cultivar ‘Qingjia #2’ and the apocarpous cultivar ‘Pinzimei’ as research meterials to study the molecular mechanism for apocapous forming. Firstly, the phenological period of two cultivars should be observed, and then we need to confirm the critical period of apocarpous by using section, and detect the mRNA expression level of AGAMOUS (AG) and WUSCHEL (WUS) by using in situ hybridization and the protein expression level of AG and H3K27me3 by Western blot, respectively. Furthermore, the occupancy of AG and enrichment of H3K27me3 at WUS should be identified by using ChIP (Chromatin Immunoprecipitation) in both two cultivars. Finally, we hope to screen differentially expressed long noncoding RNAs (lncRNAs) related to PcG by lncRNA-Seq and then present a regulatory model of AG-WUS-PcG-lncRNA in apocarpous Mei.

英文关键词： Pistil Development;Apocarpous Pistil;Transcription Factor;Histone Modification;Noncoding RNA regulation