项目名称: 基于铜配合物/石墨烯-聚苯胺修饰电极的无标记DNA电化学传感器的基础研究
项目编号: No.51273155
项目类型: 面上项目
立项/批准年度: 2013
项目学科: 一般工业技术
项目作者: 李曦
作者单位: 武汉理工大学
项目金额: 80万元
中文摘要: 基于石墨烯和聚苯胺的电子传导和比表面积的优越性,构建石墨烯-聚苯胺修饰电极,探讨修饰电极制备方法与其电化学性能之间的关系;同时基于铜的配位作用和邻菲啰啉环对DNA的插入作用以及其固有的电化学活性,将邻菲啰啉铜配合物作为修饰电极和DNA之间的连接分子来固定探针DNA,并因其具有电化学活性而作为电化学信号指示剂,以期构建一种具有高灵敏度、低检测限和宽线性范围的邻菲啰啉铜配合物/石墨烯-聚苯胺修饰电极,为无标记DNA电化学传感器的应用提供实验基础。同时,采用分子动力学和量子力学等计算方法对石墨烯/聚苯胺/邻菲啰啉铜配合物/DNA分子间的相互作用和修饰电极的电化学行为进行模拟,提出可能的电子传递和反应机理,为DNA电化学传感器的构建提供理论依据。
中文关键词: 电化学传感器;石墨烯;金属配合物;金属有机框架材料;DNA
英文摘要: A label-free electrochemical DNA sensor basing coppercomplex/graphene-polyaniline modified elctrode is designed. Firstly, graphene-polyaniline is deposited on the glass carbon electrode surface.The relationship between the prepare method of the modified eletrodes and their electrochemical properties will be studied. Then [Cu(phen)2]Cl2 ((phen = 1,10-phenanthroline) will react with the immobilized graphene-polyaniline. The immobilized [Cu(phen)2]Cl2 acts as an anchor for probe DNA immobilization and also as redox indicator.The designed label-free DNA sensor is expected to have good selectivity, sensitivity and broad detected range.For elucidating the electron transfer and reaction mechanism, quantum theory and molecular dynamic theory will be applied to simulate the action among graphene, polyaniline, copper complex and DNA
英文关键词: electrochemical sensors;graphene;metal complexes;metal-organic framework materials;DNA