拟南芥过氧化体中CHY1调控活性氧介导干旱响应的分子基础

项目名称： 拟南芥过氧化体中CHY1调控活性氧介导干旱响应的分子基础

项目编号： No.31270314

项目类型： 面上项目

立项/批准年度： 2013

项目学科： 生物科学

项目作者： 刘家尧

作者单位： 青岛农业大学

项目金额： 80万元

中文摘要： 许多研究表明，逆境因子可使植物体内积累活性氧，造成植物结构和功能伤害，甚至导致植物死亡，活性氧还是重要的信号分子参与调节植物的生长和发育。过氧化物体(Peroxisome) 与叶绿体、线粒体一样是植物细胞内产生活性氧的场所，但一直不如叶绿体和线粒体备受人们关注，尤其过氧化物体中脂肪酸氧化与活性氧、抗旱响应的关系较少涉及，因此本项目通过对过氧化物体中参与脂肪酸β-氧化的突变体chy1在干旱条件下活性氧水平、CHY1上下游代谢物、调控因子以及与过氧化物体Ca++浓度、外源蔗糖关系等分析, 结合过氧化物体中参与脂肪酸代谢的相关基因PXA1、ICL1表达分析，探讨过氧化体脂肪酸代谢与活性氧、抗旱响应的分子调控关系，指出过氧化体中CHY1参与的活性氧介导的干旱响应信号转导通路。为深入研究植物过氧化体的功能及植物抗逆分子作用机制积累资料。

中文关键词： 拟南芥；活性氧；CHY1；AtbZIP30；抗旱性

英文摘要： Many studies have shown that stress factors can make plants accumulate reactive oxygen species, resulting in the damage of the plant structure and function, and even lead to plant death. The reactive oxygen species are important signaling molecule involved in regulating plant growth and development. Peroxisomes with chloroplasts and mitochondria are the place of generation of reactive oxygen species in plant cells, but not as good as chloroplasts and mitochondria on the concern, in particular of the relationship between fatty acid oxidation and reactive oxygen species in drought-responses. This project would investigate the generation of the reactive oxygen species in chy1 mutant which is defective in fatty acid β-oxidation of peroxisome under drought conditions, as well as those of downstream metabolites, regulatory factors, peroxisome Ca++ concentration, exogenous sucrose, combined with the related genes involved in the fatty acid metabolism in peroxisome pxa1 and icl1 mutants. The study also includes analysis of the genes expression regulation and the molecular basis of the regulatory relationship between drought stress and reactive oxygen species. As results, a possible signal transduction pathway involved in CHY1-mediated oxidation and the reactive oxygen species-mediated drought response would be suggeste

英文关键词： Arabidopsis thaliana；ROS；CHY1；AtbZIP30；drought resistance