宝莲灯组培褐变的发生机制及解决对策研究

项目名称： 宝莲灯组培褐变的发生机制及解决对策研究

项目编号： No.31301800

项目类型： 青年科学基金项目

立项/批准年度： 2014

项目学科： 农业科学

项目作者： 王燕

作者单位： 浙江省农业科学院

项目金额： 23万元

中文摘要： 本项目以极易褐变的珍稀花卉植物宝莲灯为研究对象，通过子房培养获得其无菌植株材料后，观察并分析切伤对宝莲灯叶片、茎段的显微形态结构及酚类氧化物积累的时空变化分析，研究组培褐变对宝莲灯生长发育的影响方式；同时，通过对褐变前后宝莲灯表达谱的差示分析，筛选与褐变相关的基因并从分子水平上深入揭示褐变的基因调控机制；进一步地，以褐变机制研究结果为基础，通过观察和比较不同抑制剂类型及浓度对宝莲灯组培褐化的抑制效果，寻找解决宝莲灯组培褐变的对策；最后，通过对宝莲灯组培最佳增殖及生根培养基的探索，以茎尖为外植体建立组培体系。本研究将有助于全面阐明植物组培褐变的发生机制，为解决褐变危害并获得组培成功提供理论支持，对于植物组织培养研究的发展具有重要的意义。

中文关键词： 褐化；组织培养；切伤；分子机制；宝莲灯

英文摘要： The project is performed to explore the comprehensive mechanism of tissue-culture browning using the aseptic plantlets obtained by ovary cluture of Medinilla magnifica, which is a kind of rare ornamental plant and easily browns during tissue culture. Effects of browning after cutting on their growth and development will be studied by analysizing the spatial and temporal changes of cellular morphology and phenolics accumulation. And the molecular mechanism of browning will be revealed by screening browning-related genes via expression profiling analysis. Furthermore, available inhibitor of browning also will be searched based on the preceding results on browning-mechanism studies, to solve the browning response of tissue culture by comparing the effects of different types and concentrations of inhibitors. Finally, the optimal medium for proliferation and rooting will be explored in order to establish tissue culture system of Medinilla magnifica from shoot tip explants. This study will help roundly elucidate the mechanism of tissue-culture browning and provide theoretical support to preclude their jeopardizing, which also has important significance for the progress of plant tissue culture research.

英文关键词： Browning；tissue culture；cutting；molecular mechanism；Medinilla