低温高压诱导噬菌体SW1与深海细菌Shewanella piezotolerans WP3的相互作用关系研究

项目名称： 低温高压诱导噬菌体SW1与深海细菌Shewanella piezotolerans WP3的相互作用关系研究

项目编号： No.41306129

项目类型： 青年科学基金项目

立项/批准年度： 2014

项目学科： 天文学、地球科学

项目作者： 蹇华哗

作者单位： 上海交通大学

项目金额： 24万元

中文摘要： 越来越多的研究表明病毒，特别是噬菌体在深海生态系统中扮演着非常重要的角色，认识和阐明深海噬菌体与宿主的相互作用关系及机制对深入了解深海生态系统并有效地开发深海生物资源具有非常重要的意义。低温和高压是大部分深海环境的主要特点。本课题将以低温高压诱导丝状噬菌体SW1及其深海细菌宿主Shewanella piezotolerans WP3为模式系统，综合利用各种组学手段，从转录、翻译和表型三个层次上来探索低温高压条件下深海噬菌体与细菌的相互作用关系，找出在这个过程中的关键基因、蛋白质和代谢途径，构建完整的作用模型。我们将采用经典的遗传学手段和竞争性实验来对这个模型进行验证。此外，还将对分离自同一环境的病毒-宿主系统进行类比研究。最后在以上基础上，对噬菌体的关键蛋白和已有的宏病毒组进行分析，从而更准确地反映深海低温高压条件下噬菌体和宿主的演化、水平基因转移和相互作用。

中文关键词： 深海；丝状噬菌体；调控机理；噬菌体-宿主相互作用；环境适应

英文摘要： The growing numbers of studies have shown that viruses, especially bacteriophages, have played important roles in the deep-sea ecosystems, studies on the interaction between deep-sea phage and host are of great importance for thorough understanding of the deep-sea ecosystems and effective exploration of benthic biological resources. Most parts of the benthic environment of which were characterized with low temperature and high pressure. In this study, the low-temperature and high-pressure inducible filamentous phage SW1 and its deep-sea bacteria host Shewanella piezotolerans WP3 will be utilized as a modal system, and various omics methods will be applied to explore the relationship between deep-sea phage and bacterium under low temperature and high pressure conditions at transcriptional, translational and phenotypical levels, respectively. The crucial genes, proteins and metabolic pathways involved will be characterized to reconstruct the phage-host interaction model. Classic genetic means and competition experiments will be carried out to validate this model. In addition, analogy research will be performed by using the phage-host systems isolated from the same environment as SW1 and WP3. Finally, in order to accurately characterize the evolution, horizontal gene transfer and interaction between phage and host

英文关键词： deep-sea；filamentous phage；regulatory mechanism；phage-host interaction；environmental adaptation