草地早熟禾水氮互作下NRT家族基因调控机制的研究

项目名称： 草地早熟禾水氮互作下NRT家族基因调控机制的研究

项目编号： No.31501785

项目类型： 青年科学基金项目

立项/批准年度： 2016

项目学科： 农业科学

项目作者： 陈阳

作者单位： 齐齐哈尔大学

项目金额： 19万元

中文摘要： 草地早熟禾是世界公认优良冷季型草坪草，我国多用于北方城市草坪建植。氮素利用率低及干旱是制约草坪草生长及观赏质量的主要因素。挖掘固氮、抗旱基因资源，培育耐氮耐旱品种是解决问题的重要途径。植物硝酸盐转运蛋白（NRT）包含高、低及双亲和NO3-吸收转运系统，可有效调节根系吸收转运NO3-，使植物有效利用氮素。本研究采用生理和分子生物学技术，探索水氮互作中NRT家族基因调控机制。主要研究内容有草地早熟禾NRT家族基因克隆；NRT家族基因进行水氮互作下表达调控分析，初步揭示水氮通路中NRT的调节机制；NRT家族基因蛋白水氮互作下相互关系验证，揭示水氮互作中NRTs蛋白质间关系；通过转基因超量表达进行功能验证；综合分析筛选出水氮运互作既抗旱又高亲和硝酸盐转运蛋白基因。研究结果对提高草地早熟禾氮素利用效率及水分利用效率有重要意义，为完善草地早熟禾氮素营养及干旱胁迫应答机制，具有重要的研究价值。

中文关键词： 草坪草；草地早熟禾；硝酸盐转运蛋白；水氮互作；基因调控

英文摘要： Kenturky bluegrass is recognized worldwide as a good cool season turfgrass, and it's mainly used for city turf construction in northern China. Low nitrogen use efficiency and drought are the main factors which have limited the growth and ornamental quality of turgrasss. Thus, to solve the problem, exploring the nitrogen-fixation gene and drought-tolerant gene and breeding new varieties which can tolerant low nitrogen and drought conditions are important. Plant nitrate transporter protein has high, low, amphiphilic and NO3- absorb and transport system which can help the root system to absorb and transport NO3- and stimulate the plant to use nitrogen effectively. This paper studies the effects of water and nitrogen interaction on the regulation mechanism of NRT family genes through physiological and molecular biology techniques, mainly about the clone of NRT family genes in Kenturky bluegrass, the expression and regulation of NRT family genes in the water and nitrogen interaction and the preliminarily reveal of regulation mechanism of NRT family genes, the validation of mutual relationship of NRT family genes in the water and nitrogen interaction and the reveal of the relationship between NRTs proteins in the water and nitrogen interaction, function validation through over-expression in transgenic experiments, and the screen of NRT proteins which can not only tolerant drought conditions but also have high affinity through comprehensive analysis. The result of this study is of vital importance to the improvement of bluegrass's nitrogen use efficiency and water use efficiency, which provides the stress response mechanism of bluegrass to nitrogen and drought conditions.

英文关键词： Lawn;Kentucky bluegrass;Nitrate transporter;Water and nitrogen interaction;Gene regulation