周期方波电场影响蛋白质结晶的规律与机理研究

项目名称： 周期方波电场影响蛋白质结晶的规律与机理研究

项目编号： No.51201137

项目类型： 青年科学基金项目

立项/批准年度： 2013

项目学科： 金属材料学科

项目作者： 鹿芹芹

作者单位： 西北工业大学

项目金额： 25万元

中文摘要： 获取高质量蛋白质晶体是利用X射线衍射解析其分子结构的关键环节之一。因此，发展提高蛋白质结晶成功率及改善晶体质量的方法，具有十分重要的意义。申请者前期研究发现，对蛋白质结晶溶液施加周期方波电场，能提高获得蛋白质晶体的机会。该发现表明，研究周期方波电场下的蛋白质结晶，有潜在的科学意义和实际价值。因此，深入挖掘其规律及相关机理以帮助指导蛋白质结晶实践，很有必要。因溶液中结晶的驱动力是溶质的过饱和度，故可从决定过饱和度的溶质浓度场角度入手，研究其受方波电场的影响，进而研究该环境中蛋白质结晶过程的规律与机理，及该环境影响晶体质量的规律与机理，从而获得较全面的方波电场影响蛋白质结晶过程的知识。据此，申请者提出具体研究：①周期方波电场影响蛋白质结晶过程的规律与机理；②周期方波电场影响蛋白质晶体质量的规律与机理；③基于上述结果，发展新方法的可能性。研究结果将有望为发展新的结晶方法学途径提供有意义的参考。

中文关键词： 晶体质量；周期方波电场；蛋白质；形核；蛋白质结晶方法学

英文摘要： Obtaining high quality protein crystals is one crucial process for successfully determining molecular structure of proteins via X-ray crystallography, therefore developing methods that can enhance protein crystallization and improve crystal quality is of essential importance. The applicant found in previous studies that, application of a periodic square-wave electric field on crystallization solution could increase the chance to obtain crystals. This result indicated that the studies of protein crystallization under periodic square-wave electric field are potentially valuable in both scientific research and practical application. Thus it is necessary to investigate the effect of periodic square-wave electric field on protein crystallization and its mechanism. Since the driving force of crystallization from solution is the supersaturation, the investigation can begin from the study of the solution concentration distribution that determines the supersaturation. Based on the research, further investigation of the effect of the environment on the crystallization process and crystal quality and their related mechanisms can be conducted, so as to obtain a relatively full understanding of the said subject. Based on the above consideration, the applicant proposed to investigate: (1) protein crystallization process under

英文关键词： crystal quality；periodic square-wave electric field；protein；nucleation；protein crystallization methodology