用于藻毒素检测的微流体芯片的研制

项目名称： 用于藻毒素检测的微流体芯片的研制

项目编号： No.20807011

项目类型： 青年科学基金项目

立项/批准年度： 2009

项目学科： 武器工业

项目作者： 隋国栋

作者单位： 复旦大学

项目金额： 21万元

中文摘要： 经济和城市化使得我国的水污染的问题日益严重，已经开始影响居民的生活和身体健康。本项目的特点是应用于目前最新的集成化微流体芯片技术用于水中藻毒素的检测，同现行的检测技术相比具有高效、低价和高度自动化的特点。本课题所设计的微流体芯片是建立在现在经过验证的功能模块和成熟的工程手段上。藻毒素富集模块，免疫分析模块都集成到一块微流体芯片中。样品采集，富集，免疫分析，数据分析，芯片清洗（重复使用芯片）都可以通过计算机控制完成。整个过程可以设计为手动，半自动或者全自动。这种开发的技术不仅可以应用于水中藻毒素的检测，还可以直接用于其他水环境中污染物的检测，为将来建成多目标全自动检测平台打下基础。另外，这种技术不仅可以用于快速现场分析，也可以用于水体环境（包括河水、湖水和海水）中毒素的长期监测。

中文关键词： 微流控；微囊藻毒素

英文摘要： With rapid economic development and urbanization, pollution in aqueous environment in China has become a more and more serious problem, which has already affected the living and health of residents. Herein we apply the integrated microfluidics technique to the analyses of microcystins, which is high automated, more efficient and economic compared with conventional techniques. The proposed microfluidic chips are based on validated microfluidic components and engineering methods. The microcystins concentration module and immunoanalysis module are both integrated into single microfluidic chip, in which sample collection, concentration, immunoanalysis, data interpretation and self-cleaning can all be controlled by a computer. The control system can be designed as manual, semiautomatic, or automatic. The proposed technique can be applied not only for the microcystin detection, but also for the detection of other pollutants in aqueous environment, which can help to develop future automatic platform for multiple target detection. In addition, this technique can not only be applied to rapid field analysis, but also be applied in the long term monitoring of pollutions in aqueous environment, e.g. river, lake and ocean.

英文关键词： microfluidics; microcystins