基于表面分子印迹技术的超微电极传感体系及蒽醌类药物的检测研究

项目名称： 基于表面分子印迹技术的超微电极传感体系及蒽醌类药物的检测研究

项目编号： No.81503037

项目类型： 青年科学基金项目

立项/批准年度： 2016

项目学科： 医药、卫生

项目作者： 刘伟禄

作者单位： 沈阳药科大学

项目金额： 17.9万元

中文摘要： 蒽醌类抗癌药物的毒性与体内积累剂量有明显关系，控制体内蒽醌药物含量具有重要临床意义。然而建立对蒽醌药物专属性强、灵敏度高、能够活体在线检测的药物分析方法仍然是研究的难点。相比于按时间点分散式采样的分析方法，活体在线监测能更真实地表达药物的体内动态变化过程。针对此问题，本研究拟建立一种基于表面分子印迹超微电极的新型药物分析方法。以蒽醌类药物共有的蒽环结构作为“通用型”模板分子，采用电化学聚合技术，在超微电极表面制备具有特异性识别能力的表面分子印迹聚合物膜；采用石墨烯修饰超微电极以提高检测的灵敏度，微透析探针提高抗干扰能力；扫描电子显微镜技术、光谱技术、和电化学技术表征物理化学性质和特异性识别能力。优化电化学检测条件，进行方法学验证；从而建立无需样品前处理、专属性强、灵敏度高、活体在线的分析方法，并应用于生物体内多种蒽醌类药物的活体在线检测和毒性控制，为蒽醌类药物的分析提供新的思路和方法。

中文关键词： 蒽醌类药物；表面分子印迹聚合物；超微电极；石墨烯；电化学传感器

英文摘要： The determination of anthraquinone drug is clinically significant, because the drug toxicity can be caused by the accumulated dose. However, it is still difficult to develop a specific and sensitive method for determination of anthraquinone drug in vivo. Compared with time-distributed sampling and detection, the method that on-line monitoring of drugs in vivo can accurately reveal the metabolism of drugs. Therefore, an ultramicroelectrode sensing platform based on surface molecular imprinted polymer is planned. Electropolymerization method is used for preparing the surface molecular imprinted polymer with anthracene ring as the template molecule. The sensing platform is modified with graphene nanosheets and microdialysis probe in order to enhance the sensitivity and anti-interference ability, respectively. Scanning electron microscopy, spectrum technology, and electrochemical technology are used to investigate the physical property and molecular recognition ability. The detection condition is optimized. As a result, a specific and sensitive method for in vivo determination of anthraquinone is developed, which is meaningful for the development and perspective of pharmaceutical analysis method.

英文关键词： anthraquinone drug;surface molecular imprinted polymer;ultramicroelectrode;graphene nanosheet;electrochemical sensor