雨生红球藻厚壁孢子形成对虾青素合成的相关基因转录调控的影响机制

项目名称： 雨生红球藻厚壁孢子形成对虾青素合成的相关基因转录调控的影响机制

项目编号： No.31500062

项目类型： 青年科学基金项目

立项/批准年度： 2016

项目学科： 生物科学

项目作者： 万民熙

作者单位： 华东理工大学

项目金额： 21万元

中文摘要： 雨生红球藻是被誉为“抗氧化之王”的天然虾青素的最佳来源，但较低的虾青素合成效率已成为限制该产业发展的瓶颈。该藻具有多种细胞形态，而虾青素高效合成过程常伴随着厚壁孢子的大量形成。申请者发现，诱导条件相同，细胞向厚壁孢子转变的同时开始诱导虾青素合成，其合成效率远高于细胞形成厚壁孢子后的虾青素合成。因此，厚壁孢子形成对虾青素合成存在着极大影响。解析该影响是研究虾青素合成调控机制的全新切入点，有助于挖掘新的调控机制并有望用于新生产工艺的开发。本项目在前期研究基础上，拟从生理生化和基因表达层面，研究厚壁孢子形成的分子机制；进而结合厚壁孢子转变过程中虾青素合成相关基因的表达变化，从基因转录层面来阐明厚壁孢子形成对虾青素合成调控的影响机制。本项目研究不仅为基因工程改造雨生红球藻高产天然虾青素提供科学依据，而且对现有雨生红球藻光诱导合成虾青素工艺优化具有重要的指导意义，可促进天然虾青素产业的蓬勃发展。

中文关键词： 雨生红球藻；转录调控；虾青素；厚壁孢子

英文摘要： Haematococcus pluvialis is the best source of natural astaxanthin known as the king of the antioxidant, but low efficiency of astaxanthin biosynthesis has become a bottleneck of industry development on producing natural astaxanthin. In various cell morphologies of H. pluvialis, the high-efficient synthesis of astaxanthin often coupled with akinete formation. It was found in our previous study, the astaxanthin synthesis rate when astaxanthin induced at the same time of akinete formation is significantly quicker than that when astaxanthin induced after akinete formation, although the induction conditions are identical. Therefore, there is a complex relation between astaxanthin synthesis and akinete formation. The study on this relation will be a novel entry point for studying on the regulatory of astaxanthin accumulation to explore unknown mechanisms and exploit novel technologies. On the basis of our preliminary studies, the molecular mechanisms of akinete formation will be studied from cell physiology and gene expression in this project. And then, by combined with the expression difference of genes related with astaxanthin synthesis during akinete formation, the influencing mechanism of akinete formation on astaxanthin accumulation will be explored at the level of transcription. This study will not only provide a scientific basis of genetically engineered H. pluvialis for the high-efficient production of natural astaxanthin, and also be an important guide to optimize existing technologs about astaxanthin photoinduction of H. pluvialis, suggesting this project would be greatly significant for the robust development of natural astaxanthin industry.

英文关键词： Haematococcus pluvialis;Transcriptional regulatory;Astaxanthin;Akinete