基于“阴阳理论”探讨安神定志灵调节ADHD前额叶皮质DA/NE平衡状态的机制研究

项目名称： 基于“阴阳理论”探讨安神定志灵调节ADHD前额叶皮质DA/NE平衡状态的机制研究

项目编号： No.81503614

项目类型： 青年科学基金项目

立项/批准年度： 2016

项目学科： 医药、卫生

项目作者： 雷爽

作者单位： 南京中医药大学

项目金额： 18万元

中文摘要： 注意力缺陷多动障碍（ADHD）是儿童期常见的神经发育障碍疾病，但ADHD的发病机制至今尚未明确，其中讨论最热门和最能达成共识的是PFC功能障碍，以及DA和NE递质失衡理论。目前治疗ADHD的主要药物哌甲酯和托莫西汀均是通过提高PFC部位的DA和NE含量发挥作用，但是并不是对所有患儿都有效。由此推断，ADHD发病除了与DA和NE低水平有关，还存在其他失衡形式。即DA和NE处在对立统一的平衡状态，与中医阴阳理论中的“阴平阳秘”概念有相似之处。寻求古文结合本病的发病特征，本病发病与“阴阳失调”有关。中药复方安神定志灵临床疗效确切，我们拟运用微透析结合动物行为学实验，实时检测不同时间点突触间隙DA和NE递质水平的变化，以期寻找到两者的失衡状态与ADHD症状之间的联系；运用分子生物学方法，研究递质水平与影响递质水平的合成、清除、受体因素之间的关系，从而探索中药调控ADHD的可能作用机制。

中文关键词： 阴阳理论；安神定志灵；ADHD；DA/NE；平衡状态

英文摘要： Attention deficit hyperactivity disorder (ADHD) is a common neurodevelopmental disorder in childhood and adolescent and the etiology of ADHD is still unknown. The theory of PFC dysfunction and imbalance between DA and NE neurotransmitter systems has been widely accepted. Methylphenidate and Atomoxetine which are the main drugs treating ADHD by enhancing the level of DA and NE in prefrontal cortex, but they are not effective for all patients. Hence, we infer that the low level of DA and NE play an important role in the pathophysiological mechanism of ADHD, but also there exist other patterns of imbalance. There is an opposites/unity of equilibrium state between DA and NE, which is similar with the state of Yinping and Yangmi based on Yin-Yang theory. The pathogeny of ADHD is related to the imbalance of Yin and Yang according to characteristics of the disease describing in the ancient books. Anshen Dingzhi Ling (ADL) has valid clinical effects, so we intend to use microdialysis and animal behavior test to search the relationship of ADHD symptom and imbalance state of DA and NE. We also use modern molecular biology methods to study the cause of neurotransmitter imbalance, for example synthesis, clear and receptor, and how they influence the level. So we could explore the mechanism of the traditional Chinese medicine (TCM) regulating ADHD.

英文关键词： Yin-yang theory;Anshen Dingzhi Ling;ADHD;dopamine/norepinephrine;balanced state