益气活血法对4T1乳腺癌细胞肺转移及SDF-1/CXCR4生物轴的干预作用

项目名称： 益气活血法对4T1乳腺癌细胞肺转移及SDF-1/CXCR4生物轴的干预作用

项目编号： No.81503517

项目类型： 青年科学基金项目

立项/批准年度： 2016

项目学科： 医药、卫生

项目作者： 许炜茹

作者单位： 首都医科大学

项目金额： 18万元

中文摘要： 近年来研究表明SDF-1/CXCR4生物轴能激活下游通路导致乳腺癌细胞转移至特定的靶器官。而目前该轴的拮抗剂会影响正常细胞归巢到受损组织中，对免疫应答具有消极作用。因此，如何靶向性干预该轴而避免正常组织受损是目前亟待解决的课题。临床研究表明发生转移的肿瘤患者普遍存在气虚血瘀证。其病理基础是血液高凝状态，免疫功能低下。具有益气活血功效的固本抑瘤II号正是针对这一病理状态。我们的前期研究表明该方对肿瘤的转移有一定的抑制作用。本课题主要观察固本抑瘤II号对乳腺癌细胞转移至靶器官的作用，寻找该方是否通过调节机体免疫功能，改善血液高凝状态而改善肿瘤转移微环境的乏氧状态，从而对乳腺癌细胞转移密切相关SDF-1/CXCR4生物轴的调控作用，抑制了乳腺癌细胞的肺转移。为固本抑瘤II号调控乳腺癌细胞肺转移提供实验依据。

中文关键词： 肿瘤转移；乳腺癌；基质细胞衍生因子1

英文摘要： In recent years studies have shown that the SDF-1/CXCR4 axis can activate downstream pathways leading to the metastasis of breast cancer cells to specific organs and distant metastasis. However, antagonists for this axis will affect the homing of normal cells to injuried tissue and has inactive effects for immune response. Thus, how to suppress this axis exactly by avoiding the injury of normal tissue needs to be solved. Clinical studies have shown that the syndrome of qixuxueyu (qi deficiency and blood stasis) is prevalent in cancer patients with its pathological foundation of hypercoagulable and immunocompromised state.Guben Yiliu II with the effect of Yiqihuoxue (supplementing qi and promoting blood circulation) is for this pathological state. Our previous studies have shown that Guben Yiliu II has certain inhibitory effects on cancer metastasis. This research is to study the effect of Guben Yiliu II on regulating the metastasis of breast cancer cells to specific organs, and to search its mechanism. Whether this Formular can suppress the lung metastasis by regualting the immune function and hypercoagulable state, improving the hypoxia in the microenvironment leading to the inhibition of SDF-1/CXCR4 axis. It will provide the experimental evidence of Guben Yiliu II on regulating the lung metastasis of breast cancer cells.

英文关键词： cancer metastasis;breast cancer;SDF-1