石墨烯的离子束功能化修饰对其细胞和血液相容性的调控

项目名称： 石墨烯的离子束功能化修饰对其细胞和血液相容性的调控

项目编号： No.51272176

项目类型： 面上项目

立项/批准年度： 2013

项目学科： 一般工业技术

项目作者： 李德军

作者单位： 天津师范大学

项目金额： 80万元

中文摘要： 利用离子束技术实现在石墨烯上诱导形成富含氮和氧等功能基团；通过与纯石墨烯和同种工艺的功能化碳纳米管比较，理解功能基团在石墨烯上的离子束诱导构建机制，实现离子束改善和控制石墨烯对不同接触细胞和血液蛋白的构象和吸引排斥、细胞黏附与生长、细胞毒性、抑制血小板聚集，抗凝血能力等，获得不同功能基团对细胞和蛋白的识别、构象、粘附与生长的调控效果，改善细胞和血液相容性；通过实验并结合基于密度泛函的第一性原理以及生化理论，获得功能基团成分、含量、键合状态等与自由能、亲水性、电荷性质以及生物相容性之间的相互关系；根据功能基团的末端原子与接触细胞或蛋白分子中的原子在界面可能形成的化学键模型、空间群、电势等，计算模拟它们之间的相互作用，从而获得功能基团对细胞或蛋白的调控机理。该研究可以为石墨烯的应用向生物医学领域拓展提供有价值的参考。

中文关键词： 离子注入；石墨烯；细胞相容性；血液相容性；

英文摘要： The N- and O-containing functional groups are introduced to surface of graphene by ion beam method. Compared with pure graphene and carbon nanotube with functional groups prepared by identical ion beam technique, the constitution of the functional groups on the graphene induced by ion beam is understood. The functionalization graphene displays the improved cell adhesion and growth, cell toxicity, platelet adhesion and aggregation, blood coagulation, hence the improved cell and blood compatibility. The identification of cell and protein as well the control of their conformation and adhesion and growth are obtained. The first-principles and biochemical theory were used to obtain the relationship between the functional groups' compositions, contents and chemical bonding states with the free energy, hydrophilicity, charge, and biocompatibility. Based on the chemical bonding state models, space groups and potential formed by the atoms of functional groups with protein molecules at the interface, their interaction is calculated and simulated to obtain the control mechanism of functional groups to cells and proteins. This study can provide a significant reference for the expanding the biomedical applications of graphene.

英文关键词： ion implantation；graphene；cytocompatibility；blood compatibility；