水稻WRKY80转录因子基因在抗病防御过程中的调节功能与分子机制

项目名称： 水稻WRKY80转录因子基因在抗病防御过程中的调节功能与分子机制

项目编号： No.31301617

项目类型： 青年科学基金项目

立项/批准年度： 2014

项目学科： 农业科学

项目作者： 彭喜旭

作者单位： 湖南科技大学

项目金额： 22万元

中文摘要： WRKY转录因子的重要功能是调控植物抗病反应。从已鉴定功能的几个水稻WRKY基因来看，主要通过水杨酸（SA）抗病信号途径发挥调节作用，而茉莉酸（JA）在WRKY介导的抗病信号网络中的作用仍不清楚。前期，cDNA阵列和Northern杂交分析表明，水稻WRKY80受稻瘟病菌、纹枯病菌和JA诱导，而不受SA诱导，提示其可能通过JA信号途径介导水稻抗病防御。为验证此假说，本项目在分离基因cDNA序列基础上，分析基因的器官与发育阶段特异性及诱导表达模式；研究蛋白核定位、DNA元件结合和转录激活或抑制活性，以明确结构与生化功能的关系；创建超表达、RNAi转基因突变株，分析抗病性表型，以明确其生物学功能；重点分析突变株中防卫相关基因、信号通路标志基因的表达变化，鉴定互作组分，以明确其调节抗病反应的分子机理，对深入阐明水稻WRKY参与抗病防御调节及分子机制具有意义，同时可为作物广谱抗病育种提供候选基因。

中文关键词： 水稻；WRKY转录因子；基因功能；抗病信号途径；抗病防御

英文摘要： WRKY transcription factors play important regulatory roles in plant response to pathogens. So far, several rice WRKY genes have been identified to be involved in disease resistance, and most of them are associated with salicylic acid (SA)-dependent defense signal pathway. However, the knowledge on the function of jasmonic acid (JA) in the rice WRKYs-mediated defense signaling is rather limited. Previously, we confirmed by WRKY cDNA array analysis and Northern blot that rice WRKY80 was induced by blast and sheath blight causing pathogens and JA application, but not by SA application, suggesting that it might be involved in the defense response to these pathogens through JA-dependent signal pathway. To confirm this hypothesis, we focus on systematic functional analysis for the WRKY80 gene. First, we will analyze its expression profiles including expression specificity in different organs and development stages and induced expression patterns subject to various biotic, abiotic stresses and chemical treatments. Second, we will define the relationships between structural and biochemical characteristics of WRKY80 protein, including subcellular localization, DNA-binding ability and transcriptional activation or suppression. Third, to obtain the biological functions for the WRKY80 gene, we will investigate the resistanc

英文关键词： Oryza sativa；WRKY transcription factor；gene function；resistance signal pathway；disease resistance