用表界面化学控制发光纳米纤维膜的分子识别和传感

项目名称： 用表界面化学控制发光纳米纤维膜的分子识别和传感

项目编号： No.21475091

项目类型： 面上项目

立项/批准年度： 2015

项目学科： 数理科学和化学

项目作者： 周翠松

作者单位： 四川大学

项目金额： 88万元

中文摘要： 在复杂生物体系中的快速、灵敏检测是现代分析化学研究中具有挑战性的课题之一。发光纳米纤维具有比表面积大、柔韧性好及光催化能力强等优势，用于生物传感界面材料潜力巨大。本项目拟开展发光纳米纤维传感材料的表界面化学研究：探究在血液复杂体系中发光纳米纤维的表界面特征（如电荷密度、微孔结构、渗透率、发光效率等），研究高分子聚合物和发光信号分子及其表界面物理化学行为，发展发光纳米纤维的荧光信号放大技术；研究纳米纤维表面的核酸适配体组装方法及其三维构象调控手段。以癌症相关蛋白PDGF，PTK7为模型，研究核酸适配体分子层识别靶蛋白的特异性和动力学行为；探究功能化纳米纤维传感界面与传感机制间的一些规律，探索把特异性识别信号转换并进一步放大的方法，以期实现在血液复杂样品中的快速、灵敏、特异性检测。本项目的完成可为实现在复杂生物体系中的超灵敏检测提供新的方法和研究思路，在疾病早期筛查和诊断领域发挥重要作用。

中文关键词： 发光纳米纤维；生物分析；传感界面；快速响应；灵敏度

英文摘要： A fast and high sensitive detection in complex biological system is still one of challenges in modern analytical chemistry. Optical electrospun nanofiber is one of the most promising surface materials for biosensors, because of its large surface-area-to-volume ratio, good flexibility and photocatalysis capability. In this proposal, we will investigate the surface and interface chemistry of optical electrospun nanofiber that will be used as sensing materials: study surface characteristics (such as surface charge density, micropole structure, permeability, luminescence efficiency etc.) of the optical electrospun nanofiber in blood sample,study polymer and luminescence molecular and their physical/chemical behaviors on the optical fibers' surface and interface, and develop amplification methods for luminescence signal. On the other hand, we will develop modification methods of nucleic acid aptamer on nanofibers' surface, regulate 3-D conformation of aptamer modified on the nanofibers. Using cancer-related proteins (PDGF, PTK7) as targets, we will study dynamic behaviors of nucleic acid aptamer that can specifically recognize target proteins, and develop amplification methods for identification signals. Through studying fundamental problems in surface and intersurface chemistry of the optical nanofibers, we will expore some disciplines between the sensing interface and sensing mechanism of the nanofiber, for opening up new opportunities in rapid and high sensitive detection in complex biological system. In summary, this project will provide new methods and research ideas for an ultrasensitive detection in complex biological systems, and play an important role in the field of disease early screening and diagnosis.

英文关键词： Optical nanofibers;bioanalyse;sensing surface;fast response;sensitivity