六溴环十二烷对映体在蚯蚓体内的选择性富集、代谢行为研究

项目名称： 六溴环十二烷对映体在蚯蚓体内的选择性富集、代谢行为研究

项目编号： No.21307018

项目类型： 青年科学基金项目

立项/批准年度： 2014

项目学科： 环境科学、安全科学

项目作者： 田芹

作者单位： 国家地质实验测试中心

项目金额： 25万元

中文摘要： 添加型溴系阻燃剂六溴环十二烷（HBCDs）因其"持久性、蓄积性、迁移性和毒性"被斯得哥尔摩公约列为受审物质。目前，对手性HBCDs的研究主要集中在土壤、沉积物、鱼类、鸟类等异构体及其对映体污染特征以及选择性累积的检测上，而对其在土壤动物体内的富集、代谢等过程尚缺乏足够的认识。本项目以土壤动物蚯蚓为试验对象，利用手性分离技术和蚯蚓暴露模拟的试验方法，建立蚯蚓体内HBCDs异构体及其对映体的残留分析方法，通过对HBCDs异构体及其对映体在蚯蚓体内残留水平、对映体组成及可能代谢产物的检测，研究其选择性富集、转化、代谢过程，揭示HBCDs异构体及其对映体在蚯蚓体内的转化规律和代谢途径，阐明其选择性富集、代谢机理。同时通过蚯蚓微粒体体外孵育实验，探讨发生选择性代谢、转化的原因。本项目的完成对于进一步认识HBCDs的环境行为及风险性具有重要意义，为正确评价其生态健康风险以及环境污染治理修复提供科学。

中文关键词： 六溴环十二烷；蚯蚓；手性；富集；代谢

英文摘要： Hexabromocyclododecanes （HBCDs），an additive brominated flame retardant(BFRs)，are listed as a candidate for the Stockholm Convention on Persistent Organic Pollutants (POPs) because of their persistency, bioaccumulation, longrange transport, and toxicity. Currently, the study on diastereoismer- and enantiomer of HBCDs mainly focused on the selective enrichment and pollution characteristics in soil, sediment, fish, and birds. Diastereoismer- and enantiomer-specific enrichment and metabolic processes of HBCDs are still the lack of enough understanding in the soil animal. The analysis methods of diastereoismer and enantiomer of HBCDs in earthworm would be developed based on the LC-MS/MS with chiral stationary phase. Earthworms were exposed to the two concentions of individual HBCD diastereoisomer (α-,β-,γ-HBCD), to determine diasteroisomer- and enantiomer- spectific residue levels, possible degradation products and to examine the selective accumulation, degradation, and chiral signatures in earthworms. The degradation kinetics, transformation mechanism, and degradation pathway would be clarified in earthworm. Meanwile, the reasons of selective degradation and transformation would be discussed in vitro in earthworm microsomes. There would be important significance for further understanding of the environmental fate an

英文关键词： Hexabromocyclodecane；Earthworm；Chirality；Accumulation；Degradation