载紫杉醇微泡超声释放阻止乳腺癌经淋巴道转移的研究

项目名称： 载紫杉醇微泡超声释放阻止乳腺癌经淋巴道转移的研究

项目编号： No.30860269

项目类型： 地区科学基金项目

立项/批准年度： 2009

项目学科： 化学工业

项目作者： 朱梅

作者单位： 昆明医科大学

项目金额： 24万元

中文摘要： 淋巴道转移是乳腺癌的首要转移途径。由于淋巴系统特殊的解剖和生理特征，现有的治疗方法难以产生满意的疗效。已知在肿瘤周围皮下局部注射微泡并按摩，可以实现微泡超声造影剂进入引流淋巴管，进行淋巴系统超声显影。本课题按照实验计划完成了载紫杉醇脂质微泡的改良制备及评价,使其包封率及载药量相对传统的制备方法有所提高。通过肿瘤局部经皮注射按摩紫杉醇微泡进入淋巴管，联合设定强度超声辐照，使紫杉醇在淋巴管内爆破释放，杀灭或者预防经淋巴转移的癌细胞，从而达到阻断乳腺癌经淋巴道转移的目的，并探讨其作用机理。超声空化微泡释药的部分由于紫杉醇含量测定不稳定，选用Dio标记的荧光微泡代替，超声辐照爆破荧光微泡后，能有效促进荧光素在淋巴结内分布的量及范围，由此推测载药微泡经超声释放后，紫杉醇也能更多更广的分布在淋巴结及淋巴管内，从而达到更好的阻断乳腺癌经淋巴转移的局部化疗效果。

中文关键词： 乳腺癌；淋巴道；转移；微泡；超声

英文摘要： Lymphatic vessel is the primary metastatic way of breast cancer. Lymphatic system is featured with special anatomic and physiological construction, so the existing therapeutic methods could not work well on it.It is known that subcutaneous injection of microbubble around the tumour with massage could bring it into local lymphatic channels and enable contrast enhancement of lymphatic. Since the lipid-coated microbubble is a good carrier of paclitaxel, ultrasound cavitation of paclitaxel-loaded microbubbles could deliver high concentration of paclitaxel into lymphatic duct, in order to kill or prevent any metastasis of breast cancer through lymph channel.The study is based on previous successful preparation of the paclitaxel-loaded lipid microbubbles. When the paclitaxel-containing bubbles enter lymphatic by injection and massage, the bubbles could be bursted by high ultrasound pulse and deliver paclitaxel within the vessels. This might be effective in obstruction or prevention of any metastasis. So, the purpose of this study is to explore a new, less invasive, reproducible method of ultrasound drug delivery for the prevention of breast cancer metastasis.Thanks to unstability content of the paclitaxel assayed， we substituted microbubbles with fluorescence labeled by Dio for agent loaded with paclitaxel .Excited by ultrasound ,a great quantity of fluorescein were distributed in the lyphatic nodus.we conjectured that more paclitaxel could be spreaded in the lymphatic system delivered by ultrasound.

英文关键词： breast cancer；lymphatic vessels；metastasis；microbubble；ultrasound