Th17/Treg调节炎症反应与卒中后抑郁的机制研究

项目名称： Th17/Treg调节炎症反应与卒中后抑郁的机制研究

项目编号： No.81471349

项目类型： 面上项目

立项/批准年度： 2015

项目学科： 医药、卫生

项目作者： 宋景贵

作者单位： 新乡医学院

项目金额： 70万元

中文摘要： 卒中后抑郁是脑卒中患者严重的并发症，卒中后炎症反应和抑制炎症贯穿卒中后恢复的整个生理阶段，辅助性T细胞17（Th17）和调控性T细胞（Treg）对炎症双重效应起调控作用，直接影响了多种并发症的发生与否。Th17/Treg与抑郁的发生关系密切。BDNF及其相关的基因参与中枢神经系统缺氧应答，可能是卒中后到抑郁发生这一过程的子机制之一。本研究对脑卒中患者进行一年随访研究，分析患者脑功能、神经功能、Th17/Treg数量、炎症因子之间的关联，比较发生卒中后抑郁和不发生患者之间的区别，确认卒中后Th17/Treg参与调控的炎症反应与抑郁发生有关。建立大鼠卒中后抑郁模型，分析大鼠行为特征、炎症状态、脑形态以及BDNF通路基因的状况，并动态比较其在梗死后至应激形成抑郁过程中的变化，了解Th17/Treg在卒中后抑郁发生过程中的确切作用和地位，初步分析分子机制，为阐明卒中后抑郁发生的生物学机制提供依据。

中文关键词： 脑卒中后抑郁；炎症；辅助性T细胞17；调控性T细胞；脑功能

英文摘要： Post-stroke depression is a serious complication of stroke patients. Inflammatory reaction and inhibit inflammation present throughout the physiological stage of recovery after stroke. Helper T cell (Th 17) and regulatory T cells (Treg) play the dual effect on the regulation of inflammation and directly affect a variety of complications or not. Th17/Treg closely associated with the occurrence of depression. BDNF and its related genes involved in hypoxia response to the central nervous system. That is probably one of the molecular mechanisms of depression after stroke to this process. In this study, one year follow-up study will be performed in patients with of stroke. The relationships among brain function, neurological function, the numbers of Th17/Treg and inflammatory cytokines will be analyzed, and were compared between patients with depression after stroke or not. That aimed to confirm that Th17/Treg is involved in the regulation of the inflammatory response and the occurrence of depression after stroke. Then the rat model of post-stroke depression will be established, and the behavioral characteristics, inflammation, brain morphology, and status BDNF gene pathway of rats were analyzed. The dynamic changes in the process of stress depression formed in the post-infarction will be investigated to discuss the role and status of Th17/Treg in the process of post-stroke depression. The molecular mechanisms is preliminary analyzed, it may provide the basis for elucidating biological mechanisms of post-stroke depression.

英文关键词： Post-stroke depression;inflammation;Th17;Treg;brain function