毛囊干细胞microRNA调控毛乳头细胞诱导毛囊再生能力的实验研究

项目名称： 毛囊干细胞microRNA调控毛乳头细胞诱导毛囊再生能力的实验研究

项目编号： No.81472900

项目类型： 面上项目

立项/批准年度： 2015

项目学科： 医药、卫生

项目作者： 吕中法

作者单位： 浙江大学

项目金额： 75万元

中文摘要： MicroRNA是一种小的非编码非信使RNA，是动物体内主要的调节分子之一。许多研究资料表明干细胞microRNA在调控组织和器官发育方面扮演十分重要的角色。为了筛选调控毛乳头诱导毛囊再生的毛囊上皮干细胞microRNA，我们将采用激光捕捉微切技术分离毛囊上皮干细胞，用TaqMan? MicroRNA Assays建立静止期和生长期毛囊上皮干细胞的microRNA表达谱，并进行靶基因鉴定和验证；体内采用miRNA antagomir及miRNA mimics分别沉默和上调相关的microRNA，观察对毛乳头形态结构、分子表达及毛囊生物学的影响；采用经miRNA antagomir和miRNA mimics处理后毛囊干细胞分别与毛乳头共培养，取共培养的毛乳头体内进行诱导毛囊再生实验，这对阐明毛囊干细胞microRNA在诱导毛囊再生的功能调控机制具有重要的理论意义和临床应用前景。

中文关键词： 微小RNA；毛囊干细胞；毛乳头

英文摘要： MicroRNA, a small non-coding non-messenger RNA , is one of the major biological regulators which inhibits gene activity. Previous studies showed that microRNA could regulate bio-functions of stem cells, and played important roles in modulating development of tissues and organs. Our object is to identify the microRNA of hair follicular epithelial stem cells which regulate follicle dermal papilla cells-induced hair follicle regeneration. Using laser capture micro-cut technology, stem cells in different growth cycle of mice will be separated, whose microRNA expression subtypes will then be determined by TaqMan? MicroRNA Assays, in order to establish a microRNA vocabulary of hair follicular epithelial stem cells in different growth cycle of hair follicle, and the target genes are identified and tested. After treatment with miRNA antagomir or miRNA mimics, the morphology and molecular expression of the hair papilla are observed and the growth of the hair follicle is measured in vivo. The hair follicle epithelial stem cells treated with miRNA antagomir or miRNA mimics are co-cultured with hair papilla, and then the hair papilla is transplanted into skin. The ability to dermal papilla cells-inducted follicle regeneration is evaluated. We will identify the microRNA of hair follicular epithelial stem cells which regulate follicle dermal papilla cells-induced hair follicle regeneration by this study. Our study will add to clarify the mechanisms related to functional regulation of hair follicular stem cells, which is of important theoretical significance and clinical therapy prospect. So far, it has not been reported in the literature related to this study about microRNA expression in hair follicular epithelial stem cells in the different hair follicle growth cycle and regulation of function of the hair papilla that induce hair follicle regeneration.

英文关键词： MicroRNA;hair follicular epithelial stem cells;hair papilla