基于脑卒中的micro PET/CT定量与逐像素分析方法学的建立

项目名称： 基于脑卒中的micro PET/CT定量与逐像素分析方法学的建立

项目编号： No.81201114

项目类型： 青年科学基金项目

立项/批准年度： 2013

项目学科： 影像医学与生物医学工程

项目作者： 黄秋

作者单位： 上海交通大学

项目金额： 23万元

中文摘要： PET可实时活体检测正常和疾病状态下脑内的大体代谢变化。我们初步研究显示：采用microPET/CT定性和半定量方法可观察到小鼠缺血性卒中后缺血区代谢减弱及修复期血管新生诱导的代谢增强。但由于该法准确性低，无法精确定量检测到如脑卒中后内皮祖细胞移植促进血管再生，增加局部能量代谢的作用。定量（动态建模）方法准确灵敏，但目前尚无快速、无创且不受小鼠脑部功能区域内非均一性影响的算法。为应对挑战，本研究将对小鼠局灶性脑缺血模型进行动态数据采集，研究延迟显像的动态Patlak法和无需采血的参考区Patlak法，确定最优动态数据采集时段，通过静态采集数据建立小鼠脑部FDG-PET模板并进行逐像素分析。本研究提出的快速无创的micro PET/CT定量分析算法和针对非均一性的逐像素分析算法不仅为缺血性卒中研究提供切实可行高效灵敏的脑代谢检测方法，也可推广至其他疾病的microPET/CT分子影像学研究。

中文关键词： 定量；逐像素；小动物PET；；

英文摘要： Molecular imaging with the microPET is capable of observing the progression of diseases in living systems. However the qualitative analysis and Standard Uptake Value calculation wit their limitations in many applications due to the low sensitivity and low accuracy, while the conventional quantitative method requires data acquisition from the beginning of FDG injection and obtians the input function through the blood sampling. In this project we present noninvasive quantitative and voxel-based analysis with high sensitivity and high efficiency in studying the mouse stroke models. First of all, dynamic data acquisition is conducted on the Siemens Inveon microPET/CT to obtain mouse whole body images immediately after the tail vain injection of FDG. An optimal protocol together with two improved Patlak algorithms is then developed to perform fast and noninvasive quantitative studies with high sensitivity. Furthermore, a template of mouse brain is built with the static PET data to perform voxel-based studies. The methodology raised in this project is promising in answering the questions of whether Endothelial progenitor cells transplantation promotes functional angiogenesis in the study of cerebral ischemic stroke using the mouse model, and whether neuroimaging techniques provide biomarkers with high sensitivity and

英文关键词： quantification；voxel-based；microPET；；