野大麦HbCIPK2与其互作蛋白构成调控通路的功能解析

项目名称： 野大麦HbCIPK2与其互作蛋白构成调控通路的功能解析

项目编号： No.31271785

项目类型： 面上项目

立项/批准年度： 2013

项目学科： 农业科学

项目作者： 李瑞芬

作者单位： 北京市农林科学院

项目金额： 86万元

中文摘要： 在前期项目资助下，从盐生野大麦挖掘鉴定出耐盐、抗旱HbCIPK2基因，并筛选出与其互作的上游蛋白HbCBL1、4、10及下游蛋白HbSOS1和HbHAK1。但这些互作蛋白是否构成新的调控通路、调控通路又具有哪些生物学功能都还不清楚。本研究拟通过酵母突变体和拟南芥不同体系研究野大麦HbCIPK2介导的调控通路的生物学功能，确定HbCIPK2与上下游蛋白互作的关键功能域及磷酸化位点，分析HbHAK1等上下游蛋白磷酸化位点的功能、对互作的影响以及在调控通路中的作用，揭示由HbCIPK2介导的不同调控通路的作用与关系。该研究将首次鉴定不同于模式植物的野大麦HbCIPK2介导的调控通路，特别是HbCIPK2与HbHAK1调控关系的研究，将为植物抗逆调控研究提供新的遗传信息，为植物抗逆遗传改良提供新的策略和方法。

中文关键词： 野大麦；抗旱；耐盐；蛋白激酶；调控通路

英文摘要： Under supports of previous NSFC, a novel gene HbCIPK2 has been identified to be associated with salt and drought tolerance, its encoding protein interacts with upstream HbCBL1, HbCBL4 and HbCBL10, as well as downstream HbSOS1 and HbHAK1 from Hordeum brevisubulatum, respectively. However, it is still poorly understood if these interacting proteins constitute new pathways and what roles the pathways play in plant. This project will focus on the biological functions of HbCIPK2-mediated pathways through reconstitution in yeast mutant and Arabidopsis system. The aims are 1) to confirm which domains of upstream proteins (HbCBL1, 4, 10) and downstream proteins (HbHAK1 and HbSOS1) interact with HbCIPK2, and which sites of them are phosphorylated by HbCIPK2; 2) to analyze what roles these interacting domains and phosphorylated sites play in K+/Na+ homeostasis, protein interaction and the HbCIPK2-mediated pathways; 3) to dissect the functions of different HbCIPK2-mediated pathways and cross-talk between them. Based on these results, the HbCIPK2-mediated pathways different from that in model plant will be first identified in Hordeum brevisubulatum, especially, the mediation of HbHAK1 by HbCIPK2 will set up new insights into the regulation of abiotic stress in plant, and provide new ways for the improvement of abiotic stres

英文关键词： Hordeum brevisubulatum；drought tolerance；salinity tolerance；protein kinase；regulatory pathway