血小板电色谱法筛选典型活血化瘀中药活性成分研究

项目名称： 血小板电色谱法筛选典型活血化瘀中药活性成分研究

项目编号： No.81202886

项目类型： 青年科学基金项目

立项/批准年度： 2013

项目学科： 医学八处

项目作者： 杨丰庆

作者单位： 重庆大学

项目金额： 23万元

中文摘要： 抗血小板聚集是中药抗凝血作用的重要机制之一，创立以血小板为固定相的开管毛细管电色谱法能筛选活血化瘀中药中的血小板作用活性成分。本项目以细胞膜色谱和毛细管电色谱为理论基础，建立一种具有亲和毛细管电泳及细胞膜色谱双重作用机制的血小板-开管毛细管电色谱（P-OT-CEC）药物筛选新方法。结合药理学、受体学及色谱理论基础，采用阳性药及阴性药考察所建立方法的适应性、稳定性及特异性等。建立的P-OT-CEC 法应用于筛选川芎、丹参、当归、鸡血藤及益母草等中药的血小板作用活性成分，并进行体外抗凝血活性测试加以验证。同时采用峰漂移法测定活性成分与血小板的结合常数，评价结合常数与活性的相关性。通过本项目的研究建立一种高效、特异、快速的中药抗血小板聚集活性成分筛选新方法，进一步发展了亲和毛细管电泳，并拓展了药物筛选新方法在中药研究领域的应用。

中文关键词： 抗血小板聚集；亲和萃取；活性成分；作用机理；毛细管电色谱

英文摘要： Anti-platelet aggregation is one of the main mechanisms for the anti-coagulation effect of HuoXueHuaYu traditional Chinese medicines (TCMs), development of platelet based open tubular capillary electrochromatography can be used for screening of platelet active compounds from HuoXueHuaYu TCMs. Based on the theory of cell membrane chromatography and capillary electrocrhomatography,to develop a novel drug screening method platelet based open tubular capillary electrochromatography (P-OT-CEC), which has affinity electrophoretic and cell membrane chromatographic dual properties. Combined with pharmacological, receptorotical and chromatographic theories, the adaptability, stability and sepecificity of the developed method will be intensively investigated by using positive and negative drugs. Then the developed method will be applied for the screening of platelet active compounds from Ligusticum chuanxiong,Salvia miltiorrhiza, Angelica sinensis, Caulis Spatholobi and Herba Leonuri. Furthermore, in vitro platelet aggregation test will be done to confirm the platelet activity of the compounds. The binding constants for the screened out compounds will be determined by peak shift assay, and the relationship between the binding constants and their activities will be evaluated.The present study develop a effective, specific

英文关键词： Anti-platelet aggregation；Bio-affinity extraction；Active compounds；Action mechanism；Capillary electrochromatography