胰腺癌淋巴管靶向分子成像及淋巴转移机制的实验研究

项目名称： 胰腺癌淋巴管靶向分子成像及淋巴转移机制的实验研究

项目编号： No.81071195

项目类型： 面上项目

立项/批准年度： 2011

项目学科： 轻工业、手工业

项目作者： 王中秋

作者单位： 中国人民解放军南京军区南京总医院

项目金额： 10万元

中文摘要： 淋巴道转移是胰腺癌扩散的主要途径之一，在活体分子水平对胰腺癌淋巴管进行成像,可能是揭示淋巴道转移机制的关键。D2-40在淋巴管中高表达，在血管中不表达，是理想的淋巴管标记物。本项目以小鼠原位胰腺癌为研究模型，对荧光标记的胰腺癌及D2-40抗体靶向淋巴管的荧光显像、胰腺癌组织淋巴管生成状况进行研究后发现：转染绿色荧光蛋白的BXPC-3系胰腺癌及其转移灶在活体荧光影像系统上显示清楚，与离体病理检测结果一致性好；BXPC-3小鼠原位胰腺癌组织高表达D2-40，且瘤周淋巴管密度明显高于瘤内淋巴管密度，D2-40可以用作小鼠胰腺癌原位模型淋巴管标记物，同时，我们还用D2-40作为标记物研究了人胰腺癌组织淋巴管密度，发现两者的结果有相似之处；红色荧光标记的D2-40抗体能够靶向作用于皮下淋巴系统并在活体荧光影像系统显示。以上研究结果为进一步研究胰腺癌淋巴转移机制和寻找阻断其淋巴转移可能途径和方法奠定基础.

中文关键词： 胰腺癌；淋巴管；转移；荧光分子成像；分子病理

英文摘要： Lymphatic vessel metastasis is one of the important metastastic ways of pancreatic cancer. Lymphatic vessel target molecular imaging in vivo may be essential to the discovery of the mechanism of lymphatic metastasis. D2-40 is an ideal indicator of lymphatic vessels, for it has high expression in lymphatic vessels and no expression in vascular vessels. This project takes the pancreatic cancer of mouse as the research model.Fluorescence imaging for fluorescence-labeled pancreatic cancer and D2-40 targeted lymphatic vessels and lymphangiogenesis in pancreatic cancer show that the BXPC-3 pancreatic cancer and metastasis expressing green fluorescent protein are seen clearly，which are identical with pathology. The BXPC-3 pancreatic cancer express D2-40 highly and The peritumoral lymphatic vessel density was significantly higher than the intratumor lymphatic vessel density. Also, we researched the humen pancreatic cancer lymphangiogenesis, using D2-40 as label, fingding the similar outcomes. The D2-40 antibody labeling red fluorescence targeted subcutaneous lymphatic vessels of mouse, which were manifested in vivo fluorescence imaging system. The findings above of the project is to establish research basis for the discovery of the mechanism of pancreatic cancer lymphatic metastasis and the exploration of effective ways to inhibit the lymphatic vessel metastasis.

英文关键词： pancreatic cancer；lymphatic vessel； metastasis；fluorescence molecular imaging；molecular pathology.