基于插层纳米复合材料和核酸适体电化学传感器的构建及高灵敏环境激素分析

项目名称： 基于插层纳米复合材料和核酸适体电化学传感器的构建及高灵敏环境激素分析

项目编号： No.U1304214

项目类型： 联合基金项目

立项/批准年度： 2014

项目学科： 数理科学和化学

项目作者： 黄克靖

作者单位： 信阳师范学院

项目金额： 30万元

中文摘要： 复杂基质中低浓度长期暴露效应是环境激素分析的瓶颈问题，亟需建立能实现快速、特异性识别和高灵敏检测的分析方法。近来插层纳米复合材料因独特的片层结构所带来的特殊电学、催化、吸附等性能而备受关注。本课题拟以导电聚合物、有机染料、纳米金属颗粒等为客体，以过渡金属二硫化物（如MoS2、WS2等）为主体制备新型插层纳米复合材料，结合核酸适体对靶物质的特异性识别，构建新型的电化学传感器。采用底物放大、核酸分子体外扩增、标记酶催化底物放大、无机纳米粒子催化放大等电化学信号放大技术，建立环境激素高灵敏的电化学检测方法，解决水样或食品等实际样品中低浓度环境激素残留的选择性快速分析问题。构建低成本、灵敏度和重现性好的基于插层纳米复合材料-核酸适体-电化学检测的分析系统，为环境激素的毒理分析和检测评估提供新技术和新方法。

中文关键词： 环境激素；核酸适体；插层纳米复合材料；电化学传感器；信号放大技术

英文摘要： Long-term exposure and low concentration is the bottleneck problem of environmental hormone analysis in complex matrix. It is necessary to establish rapid, specific-recognition and high sensitive-detection methods for environmental hormones. Recently, intercalated nanocomposite attracted much attention for its special electrical, catalysis, and adsorption properties resulted from the lamellar structure. This project aims to synthesis of novel intercalated nanocomposites with conductive polymer, organic dye, metal nanoparticle as the guest and transition metal sulfides (such as MoS2, WS2) as the host. The novel electrochemical sensors are constructed based on the intercalated nanocomposite and aptamer, which is specific for the recognition of target substance. The signal amplification technologies including substrate amplification, nucleic acid molecular in vitro amplification, label enzyme catalyzed substrate amplification, and inorganic nanoparticles catalyzed amplification are used to develop sensitive electrochemical method for environmental hormones determination. The methods are applied to rapidly and selectively detect low-concentration environmental hormones residue in water or food samples. Low-cost, sensitive and good-repeatability analysis system is constructed based on intercalated nanocomposite-aptam

英文关键词： Environmental hormones；Aptamer；Intercalated nanocomposite；Electrochemical sensor；Signal amplification technology