云南宣威晚二叠世C1煤燃烧排放颗粒物中纳米石英、重金属元素、PAHs的细胞毒性差异及协同生物效应研究

项目名称： 云南宣威晚二叠世C1煤燃烧排放颗粒物中纳米石英、重金属元素、PAHs的细胞毒性差异及协同生物效应研究

项目编号： No.41273127

项目类型： 面上项目

立项/批准年度： 2013

项目学科： 天文学、地球科学

项目作者： 吕森林

作者单位： 上海大学

项目金额： 80万元

中文摘要： 云南宣威肺癌的高发率与晚二叠世C1煤燃烧排放污染物之间的关系一直是相关研究领域的热点,但C1煤燃烧排放颗粒物中的有害组分之间的毒性差异及其协同生物效还不清楚。本研究模拟当地居民的燃煤方式,采集C1煤燃烧排放的不同粒径颗粒物，利用气溶胶飞行时间质谱仪、高分辨透射电镜等手段对颗粒物中的纳米石英、重金属元素和PAHs进行表征,以此来研究C1煤中有害组分的迁移转化规律；通过抑制石英的表面活性、螯合重金属元素、溶解PAHs等方法获取用于细胞暴露的组分，以肺上皮细胞A549为模式，比较上述有害组分诱导细胞产生的氧化应激及其关联蛋白表达的差异，在细胞和分子水平比较它们的毒性差异并参照三元混合物(石英-Fe-苯并(a)芘)的毒性，探讨纳米石英、重金属元素和PAHs间存在的协同生物效应。研究成果能为宣威肺癌的发生提供新的科学数据，也能为煤炭资源大规模开发利用导致的环境污染健康风险的评价与预防提供科学依据。

中文关键词： 宣威大气颗粒物；燃煤排放颗粒；化学组分；氧化潜能；细胞毒性

英文摘要： The link between high lung incidence and harmful pollutants emitted from combustion of Late Permian Coal (C1) in Xuanwe, Yuanan Province has been a focus of study since 1980s. Earlier studies (Mumford et al., 1987) demonstrated that high lung cancer mortality rate was related to PAHs from combustion of C1. While a recent study (Tian,2005) has shown that nanoquartz in the coal combustion pollutants contributed to the higher lung cancer incidence, the fact that lung cancer mortalities in Xuanwei displayed stronger correlation with PSVI (the potential for silica-volatile interaction in the C1 coal) values than with volatiles or silica alone has been reported in newer research work (Large et al., 2009). Considering three toxicants (PAHs,quartz and heavy metals) are actually harmful to human, the toxicity of coal combustion-generated particles should mainly caused by them accordingly. However, so far the toxicological difference of nanoquartz, heavy metals and PAHs in Xuwei C1 coal combustion pollutants has not been well studied yet, and whether synergistic effects exist among the three toxicants remains unclear. Our study tries to solve these problems.In this study, during the laboratory experiment, we will begin with combusting collected coal in the same manner the local residents usually employed, and sample the e

英文关键词： Ambient particles in Xuanwei atmosphere；coal combustion emission；chenical components；oxidative potential；cytotoxicity