大豆GmCBL基因的eQTL分析及其功能标记开发

项目名称： 大豆GmCBL基因的eQTL分析及其功能标记开发

项目编号： No.31301341

项目类型： 青年科学基金项目

立项/批准年度： 2014

项目学科： 农业科学

项目作者： 阚贵珍

作者单位： 南京农业大学

项目金额： 20万元

中文摘要： 大豆是人类饮食和畜禽饲养重要的植物蛋白来源。尽管大豆蛋白富含大部分人体必需氨基酸，但含硫氨基酸（甲硫氨酸和半胱氨酸）含量低，限制其营养价值。GmCBL基因是甲硫氨酸生物合成路径中参与倒数第二步反应的关键酶基因，也是半胱氨酸代谢途径中的关键酶基因，在含硫氨基酸代谢途径中发挥非常重要的作用。本项目前期工作已克隆了大豆GmCBL基因，发现其过量表达确能提高转基因植物的甲硫氨酸含量。在此基础上，本研究利用大豆重组自交系群体鉴定GmCBL基因的eQTL和含硫氨基酸相关的QTL，探讨GmCBL基因表达调控机理；分析GmCBL基因的eQTL与含硫氨基酸QTL的相关性，确定关键的eQTL区间；并在大豆自然群体中对关键的eQTL区间进行关联分析，确定最优单倍型，开发与GmCBL基因表达水平紧密相关的功能标记。研究结果可为实现大豆高含硫氨基酸新品种的培育提供新的思路。

中文关键词： 大豆；含硫氨基酸；GmCBL 基因；表达数量性状位点；SNP标记

英文摘要： Soybean（Glycine max (L.)Merr.）is the important plant protein sources for human diets and livestock feedings. Although soybean protein is rich in most essential amino acids for human, its nutritional quality is limited by a low concentration of sulfur containing amino acids, methionine and cysteine. GmCBL gene codes for the key enzyme involved both in the penultimate step reaction of methionine biosynthesis pathway and in cysteine biosynthesis pathway. Therefore, it plays an important role in sulfur amino acid biosynthesis. Soybean GmCBL gene has been cloned in the previous work of this project.It was found that its overexpression can improve methionine content in transgenic plants. On this basis,this study will detect expression quantitative trait loci (eQTL) for GmCBL gene and quantitative trait loci (QTL) for sulfur containing amino acids using recombinant inbred lines population and explore mechanism for the regulation of GmCBL gene expression. Then, we will analyze correlations between eQTL for GmCBL gene and QTL for sulfur containing amino acids and determine the key eQTL interval. Finally, through the association analysis on the key eQTL interval in natural population, we will detect the optimal haplotype and develop functional markers closely linked with GmCBL gene expression. These results could provide

英文关键词： Soybean；Sulfur containing amino acids；GmCBL gene；Expression quantitative trait loci；SNP