金属植入体表面仿细胞外基质多孔胶原薄层的组装、性能和生物评价

项目名称： 金属植入体表面仿细胞外基质多孔胶原薄层的组装、性能和生物评价

项目编号： No.50872122

项目类型： 面上项目

立项/批准年度： 2009

项目学科： 生物科学

项目作者： 翁文剑

作者单位： 浙江大学

项目金额： 35万元

中文摘要： 本项目旨在金属植入体表面构建起一层既具有促进细胞附着又具有生物因子(药物)承载和缓释功能的薄层，从而使植入体能更快更好地与宿骨整合，加快修复时间和质量。研究获得主要结果为：（1）通过电化学沉积技术在Ti基板上形成矿化胶原功能薄层；（2）通过沉积电压等参数调控，可实现功能薄层的可控制备；（3）探索出矿化胶原功能层的电化学沉积机制；（4）通过温敏聚合物嵌入，进一步增强了功能涂层的生物因子(药物)承载和缓释能力；(5)通过电化学沉积技术在Ti基板上形成矿化壳聚糖涂层；（6）另外还初步开展了以TiO2纳米结构为受体，与胶原相互作用而形成功能薄层；（7）细胞培养生物评价显示矿化胶原以及嵌有温敏聚合物薄层具有良好的细胞相容性。通过上述研究完好地完成了项目预期的任务和目标。

中文关键词： 矿化胶原薄层;细胞相容性;药物承载和缓释; 金属植入体;电化学沉积

英文摘要： The project aims to build up thin mineralized collagen layers that are able to promote cell attachment and sustainably release biological factors (drugs) on the surface of the metal implants. Thus, the osseointegration would be enhanced and the success rate of implantation would be increased as well. Here are the main results: (1) thin mineralized collagen coatings on Ti substrates were obtained by electrochemical deposition; (2) the microstructure and morphology of coatings could be controlled by adjusting deposition parameters; (3) the electrochemical deposition mechanism of mineralized collagen layers was explored; (4) by embedding the thermo-sensitive polymer, the capacity of carrying and releasing biological factors (drugs) was further enhanced; (5) thin mineralized chitosan layers were also obtained by electrochemical deposition; (6) nano TiO2 was utilized as receptor that interacted with collagen to form functional bioactive coatings; (7) cell cultures and biological evaluation showed that the mineralization collagen layers and that were embedded with thermo-sensitive polymer had good compatibility. In conclusion, the expected goals were completed well.

英文关键词： thin mineralized collagen layer; cytocompatibility; drug sustained release; metallic implant; electrolytic deposition