长期土壤污染条件下蚯蚓对重金属镉毒性的整体防御体系

项目名称： 长期土壤污染条件下蚯蚓对重金属镉毒性的整体防御体系

项目编号： No.41471410

项目类型： 面上项目

立项/批准年度： 2015

项目学科： 地质学

项目作者： 乔玉辉

作者单位： 中国农业大学

项目金额： 82万元

中文摘要： 本研究采用野外调查与室内微宇宙模拟试验相结合，从个体和种群两个层面研究长期镉污染条件下蚯蚓耐性机制及生态适应性。野外调查主要研究镉污染年限、镉浓度等因素对蚯蚓种群结构和数量的影响；镉对蚯蚓全生命周期及不同世代生殖发育的影响与蚯蚓种群发育的相互关系，揭示蚯蚓种群对重金属污染的生态适应性。室内土壤微宇宙模拟试验主要研究不同蚯蚓种通过体表组织（体壁外表皮和消化道内表皮）、蚯蚓在细胞和亚细胞水平及分子生化水平上对重金属镉污染的响应，在不同生物学水平上揭示蚯蚓个体对土壤镉污染的耐性机制。在上述研究结果基础上，应用整体关联法（holistic）系统分析长期重金属污染条件下蚯蚓个体的耐性机制与蚯蚓种群水平上结构和密度变化的关系, 建立蚯蚓对重金属镉污染的整体关联性网络防御体系，为土壤污染生态风险评价和生态修复提供科学依据。

中文关键词： 蚯蚓；重金属镉；耐性机制；适应性；防御体系

英文摘要： This project will study the tolerance and ecological adaptability mechanism in long term soil cadmium contamination of earthworm individuals and population based on the field survey and laboratory experiments. The field survey will mainly study the effect of contaminated years, cadmium concentration etc. in different contaminated areas on the earthworm population structure and distribution; analyze the effect of cadmium on the development of earthworm populations from its whole life cycle and different generations to reveal the mechanism of earthworm population ecological adaptability to heavy metal contamination. With the laboratory microcosm experiments, the effect of cadmium on earthworm individual will be studied from the biological level of earthworm body wall and the digestive tract epidermis, the chloragogenous in the body cavity, enzymes and gene expression changes to reveal the mechanism of earthworm tolerance and adaptability to heavy metal contamination. Finally, the survey and microcosm experiment data will be analyzed with Holistic comprehensive methods to reveal the relationship between earthworm individuals and population and the heavy metal cadmium defense network system of earthworm will be established. This study will provide scientific data for the soil contamination risk assessment and ecological remediation.

英文关键词： earthworm;heavy metal cadimium;tolerance;adaptation;defence system