大豆GmMYB1应答植物盐胁迫的表观遗传调控及其作用机制研究

项目名称： 大豆GmMYB1应答植物盐胁迫的表观遗传调控及其作用机制研究

项目编号： No.31501328

项目类型： 青年科学基金项目

立项/批准年度： 2016

项目学科： 食品科学、农学基础与作物学

项目作者： 宋玉光

作者单位： 曲阜师范大学

项目金额： 20万元

中文摘要： 表观遗传修饰在植物逆境胁迫应答中的作用机制已成为当前的研究热点，但其在大豆盐胁迫响应中的调控机制还鲜有报道。前期研究中，我们从大豆基因组中分离到了GmMYB1基因，发现它既受盐又受DNA甲基化抑制剂诱导。盐胁迫下其启动子区DNA甲基化水平明显低于对照，过表达拟南芥株系的耐盐性明显提高，初步判断，GmMYB1通过表观遗传修饰调控参与了植物盐胁迫应答过程。本项目拟在已有研究基础上，进一步分析盐胁迫下GmMYB1的表观遗传修饰与其表达的动态调控关系；确定GmMYB1在盐胁迫响应中的功能；鉴定GmMYB1作用的下游基因，并确定其在盐胁迫应答信号通路中的作用节点；明晰大豆如何通过表观遗传修饰调节GmMYB1的表达实现对盐胁迫应答信号通路的调控。本研究有助于深入认识表观遗传调控与植物盐胁迫应答的关系，进一步拓展植物耐盐的调控理论，并为抗盐作物品种的培育提供新的思路和基因资源。

中文关键词： 大豆；表观遗传；基因表达；非生物胁迫；分子机制

英文摘要： The mechanism of epigenetic modification in response to environment stress becoming a research focus, while it’s rarely reported in soybean. In previous study, we identified a transcription factor GmMYB1, which was induced by both salinity stress and DNA methylation inhibitor. Under salt stress, the DNA methylation level within its promoter was significantly lower than the control, the over expression transgenic line exhibits a high salt tolerant ability. So we speculate, the expression of GmMYB1 may be regulated by epigenetic modification under salinity stress. Based on these study, we will further analyze the relation between GmMYB1’s dynamic epigenetic modification and expression under salt stress; confirm GmMYB1’s function; identify GmMYB1’s target genes then investigate its position in the stress regulatory network; define how GmMYB1 was regulated by epigenetic modification in the salt stress signal pathway. This study will contribute to understanding the relationship between epigenetic modification and salt stress response, extending the theory of salt tolerance in plants, providing a new theory and gene resource for investigating the mechanism of salinity response in plant.

英文关键词： Soybean;Epigenetic;Gene expression;Abiotic stress;Molecular mechanism