用于肿瘤标志物检测的稀土掺杂AMnF3（A = Na, K）单色上转换纳米荧光标记材料及其发光物理

项目名称： 用于肿瘤标志物检测的稀土掺杂AMnF3（A = Na, K）单色上转换纳米荧光标记材料及其发光物理

项目编号： No.11204302

项目类型： 青年科学基金项目

立项/批准年度： 2013

项目学科： 物理学I

项目作者： 涂大涛

作者单位： 中国科学院福建物质结构研究所

项目金额： 30万元

中文摘要： 上转换荧光标记作为肿瘤诊断的一种新技术，目前在应用上受到其材料的发射光易被生物组织吸收和穿透深度较浅的限制。本项目致力于研发一类具有单一红色（或近红外）上转换发光的稀土掺杂AMnF3（A = Na，K）纳米荧光标记材料，使其激发光和发射光均具有较高的组织穿透能力。我们将开展这类材料的可控合成、表面修饰及发光物理研究，揭示材料的上转换发光强度、能量传递过程与颗粒尺寸和稀土离子掺杂浓度的关系，阐明单色上转换发光机理，寻找提高发光效率的途径，从中筛选出一、二种高灵敏的荧光标记材料，实现对尿激酶受体（肿瘤转移的靶标分子）的上转换荧光检测，以及对肿瘤细胞的靶向成像。预期本项目研制的具有单色上转换发光特性的纳米荧光标记材料在组织穿透深度、光化学稳定性和检测灵敏度等方面都表现出优于传统有机染料和稀土鳌合物的良好性能，在肿瘤诊断、免疫分析、生物成像等领域发挥重要作用。

中文关键词： 稀土；纳米晶；上转换发光；生物标记；位置对称性

英文摘要： Emerging as a new technique for tumor diagnosis, upconversion luminescence (UCL) labeling is still limited in bio-application, due to the strong tissue absorption and low penetration depth of the emission light from upconversion materials. In this project, we will synthesize a kind of nano-biolabels based on lanthanide (Ln3+) doped AMnF3 (A = Na, K) nanocrystals (NCs) that emit single band UCL in the red or near-infrared regions. Both the excitation and emission light of these NCs can penetrate through deep tissue. The synthesis, surface modification, and photophysics of these AMnF3:Ln3+ NCs will be systematically studied. The relationship between UCL intensity, energy transfer process of these NCs and the particle size (or the dopant concentration) will be investigated, aiming to clarify the single band UCL mechanism and improve the UCL effiviency. One or two highly sensitive nano-biolabels will be chosen and used for UCL assays of urokinase-type plasminogen activator receptor (uPAR, biomarker in tumor metastasis), and for targeted imaging for tumor cells.These AMnF3:Ln3+ NCs that emit single band UCL are expected to show higher tissue penetration depth, better photochemical stability and lower detection limit than traditional organic fluorophores and quantum dots, therefore could have promising biological appl

英文关键词： Lanthanide；nanoparticles；upconversion luminescence；biolabel；site symmetry