Remorin 在玉米天然免疫反应中的调控作用及机制研究

项目名称： Remorin 在玉米天然免疫反应中的调控作用及机制研究

项目编号： No.31471503

项目类型： 面上项目

立项/批准年度： 2015

项目学科： 食品科学、农学基础与作物学

项目作者： 吴刘记

作者单位： 河南农业大学

项目金额： 85万元

中文摘要： 玉米病害是影响玉米产量和质量的主要因素之一，开展玉米免疫学研究，从而提高玉米自身的免疫力，是从根本上解决这一问题的重要途径。Remorin是植物专一性蛋白，我们前期的研究工作揭示了Remorin在玉米免疫反应中可能发挥着重要的功能。本项目是在申请人以前研究工作基础上进行的学科交叉研究，围绕研究目标主要开展以下研究内容：（1）分别从基因和蛋白水平研究病原相关分子模式对Remorin的影响，探索其在玉米免疫反应中的调控作用；（2）构建Remorin反义及超表达载体遗传转化玉米，分别从玉米免疫反应参数和抗病表型的变化来证实Remorin的功能；（3）以转基因植株为材料，利用酵母双杂交及免疫共沉淀等技术，研究Remorin的相互作用蛋白及所在信号网络关系，初步阐明Remorin的免疫作用机制。本项目旨在为进一步揭示植物Remorin的功能及作用机制奠定基础，同时对推动玉米免疫学研究有着重要意义。

中文关键词： 玉米；Remorin；免疫反应；功能分析；作用机制

英文摘要： Maize disease is one of the the main factors that affect the yield and quality of maize. Study of maize immunology will promote capacity of maize immunity, and solve maize disease. Remorin is one kind of the proteins which are specially expressed in plants. Our preliminary results suggest that remorin may play an important immunological function in maize. Based on our previous research work, this project is proposed to study remorin in maize through disciplinary pathway. The following experiments will be conducted to obtain our research target: (1) Study the effects of pathogen associated molecular patterns on Zm-Remorin through gene and protein investigation. This will explore the role of Zm-Remorin in the immune response of mazie. (2) Construction of the antisense and overexpression vectors to carry out maize genetic transformation and examine the immune response parameters and disease resistance of maize to further confirm the function of Zm-Remorin. (3) By using transgenic plants, yeast two hybrid technology and co-immunoprecipitation assay will be performed to investigate the signal network and interaction of Zm-Remorin. These results will clarify the functional mechanism of Zm-Remorin in the immune response. Conduction of this project will futher reveal the function and mechanism of plant remorin. Meanwhile, this project is of great significance in the field of maize immunology.

英文关键词： Maize;Remorin;Immune response;Functional characterization;Mechanism