核酸适体修饰的亲水超高交联整体柱电色谱高效分析痕量双酚A的研究

项目名称： 核酸适体修饰的亲水超高交联整体柱电色谱高效分析痕量双酚A的研究

项目编号： No.21277026

项目类型： 面上项目

立项/批准年度： 2013

项目学科： 环境科学、安全科学

项目作者： 林旭聪

作者单位： 福州大学

项目金额： 80万元

中文摘要： 以双酚A为研究对象，从功能材料和分析模式着手，创新研发新型核酸适体修饰的亲水超高交联整体柱及其亲和电色谱在线特异富集、分离的新模式，致力于新型有机聚合整体柱高效富集、特异识别的研究。项目首次将核酸适体亲和作用引入亲水超高交联整体柱，在高相比的超高交联聚合结构表面修饰双酚A的核酸适体，形成大量的核酸适体高特异性亲和作用位点，大幅提高整体柱亲和识别特性，研发出特异识别的高相比亲和整体柱；联用电色谱高效分析技术，建立集电场堆积富集、高相比固定相吸附和亲和电色谱分离为一体的新型电色谱在线微分析模式，解决常规聚合整体柱分子识别作用相比较小、富集效率较低、样品富集区带展宽明显等瓶颈问题，建立痕量双酚A的高特异性识别和高效分离技术。在此基础上，集成激光诱导荧光柱上检测模式，实现环境中痕量双酚A的超高交联整体柱核酸亲和电色谱高灵敏特异亲和分析。拟发表高水平论文5-8篇、发明专利2-3项。

中文关键词： 核酸适配体；亲水高交联整体柱；特异识别；双酚A；

英文摘要： For improving the efficiency and specificity of molecular recognition on the organic polymerized monolith, the researchs on novel aptamer-modified hydrophilic hypercrosslinked monolith and new model for the on-line specific enrichment and separation with affinity capillary electrochromatography (CEC) would be done by using bisphenol A (BPA) as the representative object. By means of the surface modification of aptamer on the skeleton of hydrophilic hypercrosslinked monolith, the microstructures and characteristics of the monolith will changed and a desired functional monolith will be perfect. Numbers of active sites for highly specific affinity chromatography are expected, and specific identification of BPA on this hydrophilic hypercrosslinked monolith will be also obtained. With the comprehensive application of EOF and auxiliary pressure for the continuous sample injection, the analytic technique and separation behavior of CEC with the functional monolith will be investigated. A novel on-line micro-analysis method is investigated, and several significant advantages including the enrichment of electric field-amplified action, adsorption of functional stationary phase, and the high-efficient separation of affinity CEC, will be obtained. The problems existing in the traditional enrichment mode of organic polymerize

英文关键词： nucleic acid aptamer；hydrophilic hyper-cross-linked monolith；specific recognition；bisphenol A；