项目名称: 基于量子点标记物的电化学发光免疫分析新体系和新方法研究
项目编号: No.81072336
项目类型: 面上项目
立项/批准年度: 2011
项目学科: 电工技术
项目作者: 郭智勇
作者单位: 宁波大学
项目金额: 10万元
中文摘要: 电化学发光免疫分析是新一代标记免疫分析技术,量子点的电化学发光于2002年初见报道。以量子点为标记物,结合磁微球技术、链霉亲和素-生物素技术的电化学发光免疫分析体系与方法罕见报道。本项目基于量子点和其他纳米材料,构建了超高灵敏度检测肿瘤标志物AFP的电化学发光免疫分析新体系和新方法,检测限达到20 ag/mL,是目前报道过的最高灵敏度。首次报道电化学发光-分子印迹固相萃取技术,开发了一系列检测四环素、孔雀石绿、三聚氰胺、酚酞等小分子的高灵敏方法,这些研究为在后续研究中,结合核酸适配体建立高灵敏度的"准"免疫分析检测小分子技术奠定了良好基础。采用电化学合成方法,合成了量子点ZnS、CdS,并初步应用于电化学发光免疫分析新体系和新方法研究,正在采用超快伏安法研究其机理。本项目开发的肿瘤标志物电化学发光免疫传感器,为量子点和其他纳米材料的电化学发光在免疫分析及ECL免疫传感器方面的应用开辟了一种新途径。且具有高灵敏度、特异性、稳定性、重现性和再生性能,故有很好的临床应用前景。
中文关键词: 电化学发光免疫分析;量子点;肿瘤标志物;分子印迹固相萃取;生物传感器
英文摘要: Electrochemiluminescence immunoassay (ECLIA) is a new generation of labeled immunoassay technology. Electrochemiluminescence (ECL) of quantum dots was reported first time in 2002. ECLIA system and method using quantum dots as label and combining with magnetic microsphere technique and streptavidin-biotin technique, has seldomly reported up to now.In this project,several new ECLIA systems and methods were developed for the ultrasensitive determination of tumor marker AFP based on QDs and other nano materials with a detection limit 20 ag/mL, which is the most sensitive up to now. In addition, ECL-MISPE was reported first time, and a series of methods for ultrasensitive determination of tetracyclines, malachite green, melamine and phenolphthalein were developed based on it. Those studies would lay a good fundation for developing ultrasensitive quasi-immunoassay combining with aptamer. QDs ZnS and CdS were synthesized by electrochemical methods, and were applied in developing new ECLIA system and method. ECL mechanisms of them is being studied using ultrafast voltammetry. ECLIA sensors developed in this project, opened new avenues to apply QDs and other nano materials in analytical systems and ECL biosensors. Besides, those sensors exhibit high sensitivity, specificity, stability, reproducibility and good regeneration, thus has the potential to be used in clinical application.
英文关键词: Electrochemiluminescence immunoassay;quantum dots;tumor markers;molecularly imprinted solid phase extraction; biosensors