马铃薯自交不亲和抑制基因Sli的精细遗传定位

项目名称： 马铃薯自交不亲和抑制基因Sli的精细遗传定位

项目编号： No.31201257

项目类型： 青年科学基金项目

立项/批准年度： 2013

项目学科： 食品科学、农学基础与作物学

项目作者： 李颖

作者单位： 中国农业科学院蔬菜花卉研究所

项目金额： 23万元

中文摘要： 我国是世界马铃薯生产第一大国，发展马铃薯产业对保证我国粮食安全有着十分重要的意义。传统马铃薯育种基于四倍体杂交选育，育种进程非常缓慢。近些年二倍体F1实生种育种成为国际马铃薯研究的前沿，有可能替代传统的四倍体育种方式。二倍体F1实生种育种通过不断自交剔除掉有害基因培育优良自交系，进而配制优良组合。抑制自交不亲和是开展二倍体F1育种的优先条件。前人在野生二倍体材料中发现了可以抑制二倍马铃薯自交不亲和的Sli基因，并初步定位在第12号染色体。通过国际交流，我们已经得到了Sli基因的纯合材料和初步定位的分离群体。在马铃薯基因组测序完成的基础上，本项目拟对含Sli基因二倍纯合材料进行重测序，通过基因组比较分析和遗传作图，对Sli基因进行精细遗传定位。本研究为该基因的最终克隆和二倍体F1分子育种奠定基础。

中文关键词： 马铃薯；自交不亲和；自交亲和；精细定位；

英文摘要： China has become the largest potato producing country not only for potato cultivation area but also in Megaton potato production. The development of potato agribusiness plays a key role for securing the food safty in China. The traditional potato breeding is based on the tetraploidhybridization, but the breeding process was quite slow. In recent years diploid F1 breeding is becoming the front edge of international potato research, which would be an alternative approach of potato breeding. The definition of diploid F1 true seed breeding is to culture favor inbreding lines by eliminating harmful genes and to compound a vigour combination. Depression of inbreding incompatibility is the priority condition for exploring diploid F1 breeding.Previous study reportedabout the Sli gene originating from Solanum chacoense that renders diploid potatoself-compatible, which was located in the twelfth chromosome of potato. Taking adavantage of international exchange, we got the diploid homozygote of Sli gene and segregated population for primary mapping in the previous study. With the full genome of potato available, this project will re-sequence the diploid homozygote containing the Sli gene and fine-map this gene by compatible genomic analysis and genetic mapping. This research will establish the cornerstone for cloning the S

英文关键词： potato；self-incompatibilility；self-compatibility；fine-mapping；