基于Ag2S近红外量子点的活体肿瘤新生血管可视化诊疗研究

项目名称： 基于Ag2S近红外量子点的活体肿瘤新生血管可视化诊疗研究

项目编号： No.21303249

项目类型： 青年科学基金项目

立项/批准年度： 2014

项目学科： 数理科学和化学

项目作者： 李春炎

作者单位： 中国科学院苏州纳米技术与纳米仿生研究所

项目金额： 25万元

中文摘要： 肿瘤血管生成是肿瘤生长、侵袭及转移的基础。肿瘤微血管密度(microvessel density, MVD)是评估肿瘤血管生成的金标准，是反映肿瘤增殖能力、侵袭性及恶性度的重要生物学指标。如何实现原位实时高清观察MVD是目前肿瘤临床研究领域面临的一个焦点问题。近红外二区(NIR-II 1000-1400 nm)荧光成像具有高的组织穿透深度、高的空间分辨率等优点。本项目将研制具有高量子效率和高生物相容性的近红外二区Ag2S量子点，通过对其表面功能化，连接肿瘤血管特异性抗体分子及抗肿瘤血管生成药物，构建高效Ag2S近红外量子点靶向载药系统，运用于活体肿瘤新生血管的可视化诊疗研究。本项目的研究成果将有助于建立基于活体荧光成像的肿瘤血管生成诊断、治疗及药物评估的新方法，进一步拓展近红外量子点在生物医学领域的应用。

中文关键词： 硫化银量子点；近红外二区；活体成像；肿瘤血管靶向；纳米药物递送系统

英文摘要： Tumor angiogenesis is the basis of tumor growth, invasion and metastasis.Intratumoral microvessel density (MVD) is the gold standard for assessment of tumor angiogenesis and is an important biological index of the proliferative activity, invasiveness and the degree of malignancy of cancers. How to realize in situ real-time high resolution visualization of MVD is a great challenge in tumor clinical research area. Fluorescent imaging in the second near-infrared window (NIR-II 1000-1400 nm) can offer deep tissue penetration and high spatial resolution. Here, we will prepare highly photoluminescent and decently biocompatible NIR-II Ag2S quantum dots (QDs). Through surface functionalization of the QDs, linking high-affinity antibodies for targeting tumor vessels and anti-angiogenic drugs, we build a near-infrared quantum dot-based targeted drug delivery system for visual diagnosis and therapy of tumor angiogenesis. The research results will provide new approach which is based on in vivo fluorescent imaging technique for diagnose, therapy and drug evaluation of tumor angiogenesis, and further expand the applications of the near-infrared QDs in biomedical research.

英文关键词： Ag2S quantum dot；second near-infrared window；in vivo imaging；tumor vasculature-targeted；nanodrug delivery system