DNA分子机器在人血清中肿瘤标志物检测中的应用研究

项目名称： DNA分子机器在人血清中肿瘤标志物检测中的应用研究

项目编号： No.21505065

项目类型： 青年科学基金项目

立项/批准年度： 2016

项目学科： 高分子科学

项目作者： 刘静

作者单位： 临沂大学

项目金额： 24万元

中文摘要： 基于等温扩增技术的DNA分子机器由于DNA的优良可设计性和等温循环反应明显的放大作用被用于肿瘤疾病的检测，但是目前检测对象多局限于有适体的少量蛋白或核酸类的肿瘤标志物。核酸类肿瘤标志物基本上仅存在于肿瘤细胞内部，这就意味着在疾病检测过程中需要进行组织活检提取细胞等操作，检测过程对病人创伤比较大，不利于肿瘤疾病的早期、广泛筛查。而目前创伤比较小的血清类肿瘤标志物的检测由于检测方法的局限性，灵敏度远低于核酸类肿瘤标志物。如果能将DNA分子机器放大策略应用于血清类的肿瘤标志物的检测中，有可能实现一滴血中肿瘤标志物的灵敏检测，对肿瘤疾病的早期预警及筛查具有深远意义。基于此，本项目从DNA等温循环放大策略和血清类肿瘤标志物的结合入手，开发针对血清类肿瘤标志物的高灵敏DNA分子机器传感体系，从而建立简单、高效、灵敏及准确的血清类肿瘤标志物检测技术以实现早期肿瘤的低创伤临床检测。

中文关键词： 信号放大；DNA分子机器；人血清肿瘤标志物；免疫传感器

英文摘要： DNA machine which is based on isothermal DNA amplification techniques has many advantages due to excellent properties of DNA and obvious amplification from isothermal recycling effect in detection of cancer diseases. However, the current detecting object is limited to nucleic acids which exists only inside the tumor cells. That means that the disease need to be detected after extraction of cells and other operations, which is very traumatic for patient.While,current detection of serum tumor biomarker with low level of trauma showed lower sensitivity due to the limitations of detection methods compared with nucleic acid tumor markers. If amplified DNA molecular machine stretagy could be applied to detect serum tumor markers, it is expected to achieve very sensitive detection of tumor markers. And it will has far-reaching implications for early warning and monitoring of cancer diseases. This research project intends to carry on the study of new method to detect serum tumor markers combined with isothermal DNA amplification combined cycle strategy and develope a high sensitivity sensor system for simple, efficient, sensitive and accurate clinical detection of serum tumor markers with low-trauma process.

英文关键词： signal amplification;DNA molecule machine ;human serum biomarkers;immunosensor