携带凋亡素基因的靶向结肠癌的流感病毒载体研究

项目名称： 携带凋亡素基因的靶向结肠癌的流感病毒载体研究

项目编号： No.81071849

项目类型： 面上项目

立项/批准年度： 2011

项目学科： 金属学与金属工艺

项目作者： 郭潮潭

作者单位： 浙江省医学科学院

项目金额： 10万元

中文摘要： 本项目针对我国高发的结肠癌及其治疗上存在的诸多问题，选用特异性杀伤肿瘤细胞的凋亡素为治疗药物，以转染性强、抗原性多、无致癌风险的减毒性流感病毒为载体，并对流感病毒血凝素蛋白进行定向改造，通过反向遗传技术构建出靶向性强、安全性高的抗结肠癌的减毒性流感病毒载体。同时，流感病毒载体可通过创建的抗原更换平台进行抗原更换，克服病毒载体在基因治疗中重复使用效果不理想和靶向性差副作用大的临床使用瓶颈，预期将在大肠癌的基因治疗上发挥重大作用。

中文关键词： 结肠癌；凋亡素；流感病毒；载体

英文摘要： The objective of this research project aims at the existing problem of the colorectal cancer therapy in our country. Apoptin, a tumor-selective killer, serve as antitumor drug. Attenuated Influenza virus acts as gene delivery vector with many merits, including high transfection efficiency, multiple antigenicity and no risk of carcinogenesis. Further more, influenza virus hemagglutinin gene will modified and the influenza virus vector which targeted colorectal cancer will be constructed by reverse genetic system. The vector also has reliable security. Meanwhile, the antigenicity of influenza virus vector can be exchanged with other strains by antigen change platform. In order to overcome some critical problems in clinical treatment by virus vector, such as the poor effect of reuse, poor targeting ability and the obvious side effect. We anticipate that the influenza virus vector we improved will play the significant role in colorectal cancer gene therapy.

英文关键词： Colorectal cancer; Apoptin; Influenza virus; Vector