磷在肉仔鸡小肠中的吸收规律及其分子机制

项目名称： 磷在肉仔鸡小肠中的吸收规律及其分子机制

项目编号： No.31472116

项目类型： 面上项目

立项/批准年度： 2015

项目学科： 畜牧学与草地科学

项目作者： 罗绪刚

作者单位： 中国农业科学院北京畜牧兽医研究所

项目金额： 88万元

中文摘要： 本项目拟通过肉仔鸡半体内原位结扎灌注小肠段试验、全体内自然饲喂试验和体外原代培养小肠细胞并结合基因沉默技术试验，研究磷在肉仔鸡结扎十二指肠、空肠、回肠段和原代培养小肠细胞中吸收的动力学模式及磷对自然饲喂肉仔鸡肝门静脉血浆中磷含量、正常和钠磷协同转运载体Ⅱb型(NaP-Ⅱb)、无机磷转运载体1(PiT-1)、无机磷转运载体2(PiT-2)表达分别沉默的原代培养小肠细胞中的磷转运吸收与结扎十二指肠、空肠、回肠段、自然饲喂肉仔鸡十二指肠、空肠、回肠、正常及上述转运载体表达分别沉默的原代培养小肠细胞中NaP-Ⅱb、PiT-1、PiT-1 mRNA及其翻译蛋白表达水平的影响，从而系统、深入揭示磷在肉仔鸡小肠中的吸收规律及其分子机制，丰富和发展肉鸡磷营养的现有理论和知识，对于我国肉鸡生产中如何提高其饲粮磷利用效率，节约磷资源以及减少磷排放对环境的污染，都具有十分重要的理论与现实指导意义。

中文关键词： 磷吸收；钠磷协同转运载体；基因表达；小肠；肉仔鸡

英文摘要： In the present project, series of trials half in vivo with in situ ligated small intestinal loops of broilers, in vivo with naturally feeding intact broilers,and in vitro with primarily cultured small intestinal cells of broilers in combination with the gene silence technique, will be conducted to investigate the kinetics of phosphorus (P) absorption in ligated duodenal,jejunal and ileal segments and primarily cultured small intestinal cells, and the effect of P treatment on plasma P contents from the hepatic portal vein of intact broilers, P absorptions in normal and typeⅡb sodium-phosphate cotransporter (NaP-Ⅱb),inorganic phosphate transporter 1 (PiT-1),inorganic phosphate transporter 2 (PiT-2) expression-silenced small intestinal cells in the primary culturre,and mRNA and protein expresstion levels of NaP-Ⅱb,PiT-1 and PiT-2 in ligated duodenal,jejunal and ileal segments as well as in these small intestinal segments of intact broilers, and normal and these transporters expression-silenced small intestinal cells in the primary culture, so as to systemicly and deeply elucidate absorption traits of P in the small intestine of broilers and its molecular mechanisms. The outcomes from this project will be of great theoretical and practical significances for how to improve dietary P utilization efficiency,save P resource and minimize the environmental pollution of P excreted by broilers in the broiler production.

英文关键词： phosphorus absorption;sodium-phosphate cotransporters;gene expressions;the small intestine;broilers