基于实脾法对AD大鼠GFAP、calpain的影响研究“脾主时”的分化

项目名称： 基于实脾法对AD大鼠GFAP、calpain的影响研究“脾主时”的分化

项目编号： No.81202613

项目类型： 青年科学基金项目

立项/批准年度： 2013

项目学科： 医学八处

项目作者： 蔡华珠

作者单位： 福建中医药大学

项目金额： 23万元

中文摘要： "脾主时" 是五脏应时学说的一个组成部分。文献研究表明脾与时有"脾主四时之中"、"脾主四时之末"和"脾主长夏"三种不同相应的归类，均停留在理论思辨的层次；对于其现代内涵的揭示，缺乏系统的研究。脾为气血生化之源，中医防治疾病注重"未病先防"、"因时制宜"，因此对于"脾主时"分化的研究尤为必要。申请者前期研究表明实脾法能增强AD大鼠海马中GFAP的表达；文献研究表明，钙蛋白酶(Calpain)与肌肉萎缩，及阿尔茨海默病等疾病的发生有关。因此，本研究采拟用免疫组化和生化酶学的方法，于"脾主四时之中"、"脾主四时之末"和"脾主长夏"，分别探讨运用实脾法对AD大鼠GFAP和Calpain含量变化影响的规律，进而系统对中医理论中脾与时的不同相应归类的理论内涵做出科学的解释。从而补充"脾主时"现代研究的不足和空白，完善"五脏应时"的研究内容，对临床运用"脾主时"理论指导AD的防治提供现代科学的依据。

中文关键词： 脾；脾主时；四时；长夏；阿尔茨海默病

英文摘要： "Spleen" is an integral part of the doctrine of the five internal organs seasonal. Literature studies have shown that the spleen and the spleen seasons. "," End of the spleen four seasons "and" long summer, spleen three different classification accordingly, all remain speculative level in theory; its modern the Connotative, lack of systematic research.The spleen is the source of qi and blood, the TCM disease prevention and treatment focus on "preventive treatment before" and "timely". Therefore, the "spleen" differentiation is particularly necessary. The preliminary study of the applicants that the Spleen method can enhance the AD rat hippocampus in GFAP expression; literature studies have shown that calpain (Effect of Calpain) and muscle atrophy, and the occurrence of Alzheimer's disease and other diseases. Therefore, in this study proposed using immunohistochemical and biochemical enzymatic method, "among the spleen four o'clock, spleen governs the end of the four seasons and the spleen long summer, explore the use of real Spleen AD rat GFAP and Calpain content of the law, and thus make scientific explanations of the theory of Chinese medicine spleen with different theoretical connotation of the corresponding classified. Thereby complementing the "spleen," the modern study of the deficiencies and gaps, and imp

英文关键词： spleen；spleen to time；the four seasons；long summer；alzheimer's disease