基于适配体及功能碳纳米材料的循环肿瘤细胞高灵敏检测方法研究

项目名称： 基于适配体及功能碳纳米材料的循环肿瘤细胞高灵敏检测方法研究

项目编号： No.21475085

项目类型： 面上项目

立项/批准年度： 2015

项目学科： 数理科学和化学

项目作者： 瞿鹏

作者单位： 商丘师范学院

项目金额： 50万元

中文摘要： 循环肿瘤细胞（Circulating tumor cell，CTC）检测能为癌症的筛查、复发早期预警及疗效评价等提供极具价值的科学依据，但是由于CTC在外周血中浓度极低，且血液中一些高荧光背景物质干扰其光学检测，目前高灵敏度地检测CTC仍是临床亟需解决的一大难题。本项目拟采用氧化铁掺杂法制备磁性石墨烯，以结合在其上的适配体SYL3从血液中捕获、磁分离CTC；基于适配体和适配体/CTC复合物在磁性石墨烯表面吸附能力的差异，引入酶催化信号放大策略建立CTC化学发光检测新方法；通过实时荧光定量PCR技术开发CTC荧光分析新方法；利用水热法制备高荧光量子产率的近红外碳点，进而制备多靶点碳点/磁性氧化铁纳米粒子复合材料，构建新型外周血中CTC捕获、富集、近红外荧光成像检测探针。本项目的实施可为研究肿瘤转移机制、指导肿瘤治疗、推断预后提供可靠参考，具有一定的理论意义和潜在的应用价值。

中文关键词： 稀土发光材料；碳基纳米材料；小分子荧光探针；肿瘤细胞；锂电池阴极材料

英文摘要： The detection of circulating tumor cells (CTC) can provide valuable scientific theories for screening cancer patientsrecurrence,warning recurrence and evaluating treatment, but due to a very low concentration of CTC in peripheral blood , and blood content with high fluorescence background interference their optical detection, the high-sensitivity detection of CTC is still a major clinical problem need to solve. In the present project, a novel preparation protocol of functional materials of Magnetic Iron oxide nanoparticles (MION) doped graphene are to be studied. CTCs are captured and separated from the blood using aptamers SYL3 bound onto magnetic graphene. Enzyme catalyzed signal amplification strategy based absorbance difference between aptamers and aptamers/CTC is explored for detection of circulating tumour cells. In addition, capturing CTC by aptamer, real-time quantitative PCR technology is explored for detection of circulating tumour cells with high sensitivity and good selectivity. By hydrothermal method, carbon dots having functions of near-infrared photoluminescenceand and high fluorescence quantum yield will be prepared, and then carbon dots with CTC aptamers are linked onto MION to build a novel fluorescence imaging agents for high sensitive detection of CTC. The implementation of this project has

英文关键词： Rare earth doped luminescent material；Carbon nanomaterials；Fluorescent molecular probes；Tumor cells；Lithium-ion battery cathode materials