杏鲍菇免疫调节蛋白生物活性及其作用机理

项目名称： 杏鲍菇免疫调节蛋白生物活性及其作用机理

项目编号： No.31471927

项目类型： 面上项目

立项/批准年度： 2015

项目学科： 农业科学

项目作者： 胡秋辉

作者单位： 南京财经大学

项目金额： 90万元

中文摘要： 食用菌中富含的多种功能成分可以促进国民健康,杏鲍菇是近年国内生产和消费量最大的食用菌之一。目前对杏鲍菇富的免疫增强、抗肿瘤、抗炎症等多糖、蛋白功能成分的作用研究较多，对杏鲍菇蛋白的结构、免疫功能作用机制、蛋白表达特性及其表达机制的研究还未见报道。本项目在申请者前期杏鲍菇功能作用评价的基础上，基于品种改良基础理论及蛋白特性和蛋白生物化学解析研究，筛选不同品种和制备杏鲍菇免疫活性蛋白，解析杏鲍菇免疫活性蛋白组分的化学结构。研究杏鲍菇蛋白对免疫缺陷动物模型和细胞模型系统生理生化指标调控、细胞因子水平表达和对NF-κB细胞通路的调控作用。从动物整体水平和细胞群体水平解释杏鲍菇蛋白的免疫调节作用机制，阐明杏鲍菇蛋白免疫调节功能作用的构效关系，明晰杏鲍菇免疫蛋白表达特性及其作用机制，为该蛋白高表达品种选育和表达调控机制等研究奠定基础。

中文关键词： 杏鲍菇；蛋白；免疫调节；构效关系；作用机理

英文摘要： Edible fungi have high content of functional compounds, which can promote public health. Currently majority of researches are focusing on the polysaccharides and proteins rich in Peurotus eryngii (mushroom), which are proved to have immune enhancement, anti-tumor and anti-inflammatory activities. However, there is few studies about the protein structure, the mechanism of immune activity, the protein expression properties and mechanisms of the Peurotus eryngii protein. Based on our previous studies, this project aims to investigating the chemical composition of bioactive protein from Pleurotus eryngii and analysis the protein structure using modern instrumental techniques. We will study the role of bioactive protein from Pleurotus eryngii in the regulation of immune systems in the immunodeficiency animal mode and in vitro cell mode as well as its impact on the in vivo levels and expression of cytokines and on the regulation of NF-κB pathway. In combination of data from in vitro cell culture studies and animal experiments, we will explore the effect of bioactive protein on the immune systems to reveal the relevant mechanism of immunoregulatory function of the bioactive protein from Pleurotus eryngii and clarify its structure-function relationship using established Quantitative models. This study will provide a scientific basis for breeding new variety of high expression of Pleurotus eryngii protein and researching on the expression regulation mechanism of the special protein.

英文关键词： Pleurotus eryngii;Protein;Immunomodulatory;Relationship of structure and bioactivity;Mechanism