癌症非编码miRNA-lncRNA协同调控网络重构与功能特性研究

项目名称： 癌症非编码miRNA-lncRNA协同调控网络重构与功能特性研究

项目编号： No.61473106

项目类型： 面上项目

立项/批准年度： 2015

项目学科： 自动化技术、计算机技术

项目作者： 李霞

作者单位： 哈尔滨医科大学

项目金额： 82万元

中文摘要： lncRNA是一类长度超过200nt的非编码RNA，在生命活动的调控网络和复杂疾病的发生发展过程中都扮演重要的角色。系统识别复杂疾病相关lncRNA和miRNA，揭示LncRNA与miRNA协同调控机制是具有挑战意义的工作。本课题从系统生物学的角度，整合lncRNA和miRNA二代测序数据、miRNA和lncRNA芯片数据、miRNA及靶基因双重表达谱和蛋白谱等高通量数据，挖掘疾病关联的lncRNA与miRNA，并重构miRNA-lncRNA协同调控网络，探索复杂疾病非编码miRNA-lncRNA的协同作用机制和网络调控特点，建立复杂疾病miRNA-lncRNA及miRNA-lncRNA-mRNA协同调控功能模块识别的生物信息学分析策略与分析平台，并应用于胶质瘤、乳腺癌、肺癌等复疾病的研究中，挖掘出的疾病特异lncRNA和miRNA-lncRNA协同调控关系为疾病的机制研究提供重要依据。

中文关键词： 恶性肿瘤；非编码RNA；高通量测序；协同调控；miRNA-lncRNA

英文摘要： Long non-coding RNAs （lncRNAs） are a class of non-coding RNA molecules with the length of more than 200 nucleotides. They have been shown to play crucial roles in regulatory network of life activities and the development of complex diseases. Systematic identification of lncRNAs associated with complex diseases and clarifying synergistic regulation mechanisms with miRNA are still challenging. Integrating multiple high throughput data are chosen in this research, such as next generation sequencing data of lncRNA and miRNA, lncRNA array data, miRNA expression profiles, gene expression profiles and protein expression profiles. Meanwhile, a system biology approach is used to identify risk lncRNAs and miRNA associated with complex diseases and construct miRNA-lncRNA synergistic network, also, exploring synergistic regulation mechanisms and network regulatory features of non-coding RNA, furthermore, establishing Bioinformatics Analysis Approach and Platform for identifying disease lncRNAs,miRNA and regulating functional modules of miRNA-lncRNA synergistic network in complex disease. The disease related lncRNAs and functional modules in synergistic regulation would be identified in our research, that can be used to not only do further study the mechanisms of complex diseases, for example, contains Glioma, breast cancer, lung cancer, liver cancer and so on, but also provide novel insights and drug target for disease diagnosis and therapeutics. This study has important scientific and clinical value in assisting with future studies of non-coding RNA synergistic regulation and complex disease pathogenesis.

英文关键词： cancer;non-coding RNAs;high throughput sequencing data;synergistic regulation;miRNA-lncRNA