民族药四方藤抗炎有效成分的分离、活性筛选及作用机制研究

项目名称： 民族药四方藤抗炎有效成分的分离、活性筛选及作用机制研究

项目编号： No.21262006

项目类型： 地区科学基金项目

立项/批准年度： 2013

项目学科： 数理科学和化学

项目作者： 覃江克

作者单位： 广西师范大学

项目金额： 50万元

中文摘要： 四方藤是广西、云南等少数民族地区用于风湿痹痛、十二指肠出血、胃炎等炎症相关疾病治疗及外伤止血的民间常用植物药，其来源丰富、价格低廉、疗效广泛而显著。本项目以抗炎、抗氧化等药理活性筛选为指导，利用脂多糖（LPS）刺激巨噬细胞建立体外炎症模型和细胞氧化应激等方法，以各种层析、波谱等现代提取分离和结构测试手段确定四方藤有效部位的化学成分，明确其有效的抗炎组分，筛选出兼具有良好抗氧化和抗炎活性的成分；对于筛选到的药效显著、具有良好发展前景的化合物，应用酶联免疫吸附、RT-PCR、Western blot等分子生物学和药理学的研究手段，在细胞和分子水平上探讨化合物发挥抗炎活性的可能机理。通过四方藤化学成分的提取分离、结构鉴定、活性筛选和作用机制的研究，为民族药四方藤的深度研究与开发利用提供理论依据和实验指导。

中文关键词： 四方藤；化学成分；抗炎；抗氧化；抗黑色素生成

英文摘要： Cissus Pteroclada Hayata is one of the characteristic nature medicinal plants belonging to the family of Cissus Linn.of Vitaceae, which is widely distributed in south China such as Guangxi, Yunnan province. As a traditional anti-inflammatory drug, it is used to treat rheumatic diseases, duodenal hemorrhage, gastritis etc. in Chinese folk with nice curative effects. In this project, a series of chromatography and spectrum methods are applied to separate and identify the chemical constituents from the effective fractions of Cissus Pteroclada Hayata monitored by LPS-stimulated macrophage and oxidative stress model in vitro based on anti-inflammatory and anti-oxidant activity. This study aims to indentify the anti-inflammatory constituents of Cissus Pteroclada Hayata, especially those with both nice anti-inflammatory and anti-oxidant activities. For the compounds which possess excellent anti-inflammatory effect, pharmacological means such as ELISA, RT-PCR,Western Blot will be applied to explore their possible anti-inflammatory mechanisms at the molecular and cellular level. In a word, the task of this research is to explore the chemical constituents of Cissus Pteroclada Hayata through extraction, separation and structural characterization, as well as their pharmarcological activites and the possible mechanisms. This

英文关键词： Cissus Pteroclada Hayata；chemical constituent；anti-inflammatory activity；antioxidant activity；inhibition of melanin synthesis