可控分子元件的DNA自组装模型与应用研究

项目名称： 可控分子元件的DNA自组装模型与应用研究

项目编号： No.61272246

项目类型： 面上项目

立项/批准年度： 2013

项目学科： 自动化技术、计算机技术

项目作者： 董亚非

作者单位： 陕西师范大学

项目金额： 80万元

中文摘要： DNA纳米技术不仅为纳米元件的制造和分子级电子元件的研究提供了新的方法和技术，也在DNA计算机、纳米生物机器及基因治疗等方面有特殊的应用价值。分子自组装模型以生物分子及其相关酶为基本材料，通过可控的生化反应来构建分子元件，进而完成信息传递与处理。本项目以当前纳米金颗粒技术、链置换技术及生物芯片技术的发展为基础，对分子元件的自组装模型构建及其应用进行理论和实验研究。主要通过以下方面实现：⑴拟基于分子自组装、DNA链置换技术和生物芯片技术理论构建分子元件，构造一种可执行信息传递与处理功能的生物分子元件或自组装基元；⑵拟通过DNA自组装基元与分子元件的编码设计，研究分子元件在肿瘤细胞周期中的生物信息传递与处理能力, 探讨分子元件通过构建基因网络处理信息的能力及生物计算问题；⑶拟通过分子元件构建可控的自组装模型，利用纳米金颗粒、磁珠、凝胶电泳、链置换和荧光标记等技术，进行生化实验验证。

中文关键词： DNA自组装；可控的；逻辑门；分子元件；生物计算

英文摘要： DNA nanotechnology can not only provide new approach and technology for manufacturing nanodevice and study of electronic device in molecular level，but also has its particular application value in DNA computer ，nanobiological machine, gene therapy and so on . Using biological molecular and related enzyme as elementary material, molecular self-assembly models can construct molecular device through controllable biochemical reaction to implementing transfer and process of information . Based on the development of AuNPs , strand displacement and biochip technology , the project covers both theory and experiment research of designing model for molecular device self-assembly and its application. It can be implemented through the following ways ： ⑴we intend to construct a bio-devices or self-assembly primitive can be used to perform information transmission and processing based on the technological theories of molecular self-assembly, DNA strand displacement and biochip. ⑵we expect to expand the application of molecular device in the information transmission and processing via the routes of programming and designing DNA self-assembly primitive and molecular device , and explore the ability of molecular device by c

英文关键词： self-assembly；controllable；logic gates；molecular element；biological information