磷脂分子的电场诱导可控组装及组装体携载性能研究

项目名称： 磷脂分子的电场诱导可控组装及组装体携载性能研究

项目编号： No.21503072

项目类型： 青年科学基金项目

立项/批准年度： 2016

项目学科： 数理科学和化学

项目作者： 毕洪梅

作者单位： 黑龙江八一农垦大学

项目金额： 21万元

中文摘要： 磷脂是生物膜最重要的组成成分，在生物体中主要呈类似于细胞基质框架的球形结构和中空的管状结构两种状态。在体外进行磷脂组装体的简单、快速、可控组装并进一步在载药、物质转移、生物反应等方面进行深入研究，是当前许多研究领域重点关注的课题。本项目以共平面叉指微电极进行电场下的磷脂组装体可控组装研究，并对不同条件下的电场分布进行模拟与计算分析，明确控制组装体形态变化的关键因素，揭示电场的调控机理。结合磁性纳米粒子，利用电场调控诱导形成具有球形头部及链状尾部的磁性载药“磷脂蝌蚪”，并研究其在静磁场和交变磁场双向磁场作用下的运动及药物释放。进行在生理盐度下磷脂囊泡的电场诱导组装研究，并以组装的巨型磷脂囊泡为细胞模型进行磷脂囊泡内的聚合酶链反应（PCR）。本研究预期结果为构建安全高效的靶向给药载体、解决体外生物反应的难题、扩大磷脂在生物医学的应用等研究提供新方法和新技术。

中文关键词： 叉指微电极；可控组装；机理；靶向给药；PCR

英文摘要： Phospholipid is the most important component of biological membrane, it mainly exist as the spherical structure similar to cell framework and hollow tubular structure in vivo. Nowadays, it is urgent and important issue for many research groups to establish a simple, fast, controllable assembling method of phospholipids in vitro, further develop the application in drug delivery and biological reaction using these assemblies. The interdigitated microelectrodes were used in this project to study the controllable assembling of giant unilaminar vesicles and lipid tubes in electric field. The distribution of electric field above these interdigitated microelectrodes in different conditions were simulated, calculated and anglicized in detail. Base on the experimental and theory results, the critical factor for the morphology control of phospholipid assembly was clarified and the regulatory mechanism was proposed. Moreover, combination with magnetic nanoparticles, the magnetic and drug “lipid tadpoles” with spherical head and long tail were assembled using the special electric field of interdigitated microelectrodes. The motion and drug release of these “lipid tadpoles” were investigated in vertical static and alternating magnetic fields. The studies of phospholipids controllable assembling under the physiological salinity and polymerase chain reaction in giant unilaminar vesicle were also developed. The prospective results of this project aim to provide a novel method and novel technology for the study of fabrication of an safe efficient targeted drug carrier, solving the puzzle of biological reaction in vitro and expanding applications of phospholipids in biomedical.

英文关键词： Interdigitated microelectrodes;Controlled assembly; Mechanism;targeted drug delivery;Polymerase chain reaction