低活力精子miRNA表达谱的建立及其对精子发生的影响

项目名称： 低活力精子miRNA表达谱的建立及其对精子发生的影响

项目编号： No.31071317

项目类型： 面上项目

立项/批准年度： 2011

项目学科： 轻工业、手工业

项目作者： 陈德宇

作者单位： 阜阳师范学院

项目金额： 8万元

中文摘要： 应用基因芯片技术构建弱精症患者精子中miRNA表达谱。收集30份弱精症患者精液标本和20份成年健康有生育能力男性的精液标本，提取精子RNA，标记后，与miRCURYTM Array芯片杂交，分析弱精症患者精子中miRNA表达情况。高活力精子和低活力精子共196个miRNA存在表达差异，上调miRNA 93个，下调miRNA 103个；制备生物素标记的cDNA探针，与含有30 968个探针的Phalanx OneArrayTM芯片杂交，分析基因表达情况。结果表明高活力精子和低活力精子共有1995个基因存在表达差异，上调基因有394个，下调基因有437个。弱精症患者精子miRNA和mRNA表达谱的建立对分析精子运动的分子机制和探讨弱精症的病因将会有所帮助。

中文关键词： 弱精症；miRNA;mRNA;精子；表达谱

英文摘要： To construct and analyze the miRNA and mRNA profiles in asthenospermia patients’sperm. 30 semen samples from asthenospermia patients and 20 semen samples from healthy normal adult men were collected, respectively, and then to extract the total RNAs and to produce probes. Hybridization with miRCURYTM Array was carried out. 93 miRNAs were up-regulated, 103 miRNAs were down-regulated.Hybridization with Phalanx OneArrayTM contained 30 968 probes was carried out after the labeled cDNAs pured by PCR Product Purification Kit.Among the 30 968 probes, 394 genes were up-regulated, 437 genes were down-regulated.Construction of miRNA and mRNA profiles in asthenospermia patients’sperm can help to analyze the molecular mechanism of sperm motility and to study the etiopathogenisis of asthenospermia.

英文关键词： asthenospermia；miRNA;mRNA;sperm;profile