基于二硫化钼纳米生物传感器检测微系统的构建及其在肺癌早期检测中的应用研究

项目名称： 基于二硫化钼纳米生物传感器检测微系统的构建及其在肺癌早期检测中的应用研究

项目编号： No.21475034

项目类型： 面上项目

立项/批准年度： 2015

项目学科： 数理科学和化学

项目作者： 张国军

作者单位： 湖北中医药大学

项目金额： 85万元

中文摘要： 本项目面向肺癌早期检测的重大需求，构建新型二硫化钼场效应晶体管生物传感器，初步建立一套基于该生物传感器的sample-in-answer-out的检测微系统用于肺癌早期检测。采用液相剥离法大规模制备二硫化钼纳米材料，将这种纳米材料转移至二氧化硅表面，在二硫化钼表面用一层金膜覆盖，再用导电银胶电极制作源极和漏极，得到免印刷术(lithography-free)制备的新型纳米生物传感器器件。研究蛋白质分子在二硫化钼表面功能化的新方法，优化抗原-抗体相互作用的条件，建立基于微过滤装置和二硫化钼纳米生物传感器相结合的检测微系统，并利用该检测系统直接从手指血中同时检测与肺癌相关的3种蛋白质标志物CEA、CA-125和cyfraz-1。与此同时，实施该系统对肺癌病人样品的检测，用现有的临床方法判定检测结果，进行方法评价。本项目的确立将为新型纳米生物传感技术在肿瘤早期检测领域提供新方法。

中文关键词： 生物传感器；纳米材料；生物纳米技术；免疫分析；生物分析化学

英文摘要： Driven by the significant demand of early detection of lung cancer, this project is to fabricate a novel MoS2-based field-effect transistor (FET)biosensor and construct a sample-in-answer-outdetection microsystem based on MoS2 FET biosensor for early detection of lung cancer. MoS2 nanosheet is largely prepared by liquid exfoliation process, and transferred to the surface of SiO2. The MoS2 FET biosensor is then fabricated in a 'lithography-free' manner by depositing a Au film on the MoS2 surface and subsequently making conductive Ag paste as electrodes for electrical measurements. The functionalization method of immobilizing protein on the sensing surface is investigated and the interaction between antibody-antigen is also optimized. The detection microsystem is then obtained by combining a microfilter setup with the MoS2 FET biosensor, which is used for multiplexed detection of lung cancer-related 3 protein biomarkers including CEA, CA-125 and cyfraz-1 from finger prick blood. Meanwhile, the clinical blood samples from the lung cancer patients are investigated by the detection microsystem. The detection results are evaluated by the classical method used in clinical examination. The determination of the project will pave a new way for application of new nano-sensing technologies in tumor early detection.

英文关键词： Biosensor;Nanomaterials;Bio-nanotechnology;Immuno-analysis;Bioanalytical Chemistry