基于Ag(III)的化学发光新体系在毛细管电泳-化学发光法检测食品中霉菌毒素中应用和发光机理研究

项目名称： 基于Ag(III)的化学发光新体系在毛细管电泳-化学发光法检测食品中霉菌毒素中应用和发光机理研究

项目编号： No.81502846

项目类型： 青年科学基金项目

立项/批准年度： 2016

项目学科： 医药、卫生

项目作者： 马莉

作者单位： 河北医科大学

项目金额： 18万元

中文摘要： 霉菌毒素品种多, 污染广, 毒性强, 危害重，实现霉菌毒素的快速、准确、灵敏的分析是食品安全领域的一项重要研究课题。而现有的检测方法存在仪器昂贵，检测成本高，费时等不足，难于普及和满足市场监督的需求。早期研究发现，多种物质都对Ag(III)-鲁米诺化学发光体系有增敏或抑制的作用。近期研究发现Ag(III)配合物作为一种强氧化剂，自身在酸性介质和碱性介质中可氧化某些毒素并产生化学发光。据此，建立毛细管电泳-化学发光检测毒素的新方法和研制毒素快速检测技术，实现食品中霉菌毒素快速、准确、灵敏检测。本课题拟研究：（1）探索Ag(III)配合物分别在碱性鲁米诺溶液，硫酸溶液和氢氧化钠溶液中与相关毒素的化学发光反应机理。（2）建立基于Ag(III)化学发光新体系的毛细管电泳-化学发光法，实现粮食中伏马毒素，黄曲霉等毒素准确快速测定。（3）研制免疫亲和柱-化学发光法检测相关毒素的快速检测技术。

中文关键词： 霉菌毒素；毛细管电泳-化学发光；Ag(III)配合物；机理研究

英文摘要： Mycotoxins are characterized by more varieties, wide polluted, heavy toxicity and great harm. The fast, accurate and sensitive detection method of mycotoxin, one of a critical research area in food safety, is an important base for the evaluations of food health. Previously reported methods have some drawbacks including expensive instrumentation, high-cost and time-consuming. They are difficult to be widely used and to meet need of market supervision. In our early studies, we found many compounds have effects of enhancement or inhibition on chemilulminescence signal in Ag(III)-luminol system. In recent studies, Ag(III) complex as a strong oxidant can produce strong chemiluminescence signal in acid or alkaline medium. Based on this findings, we intend to establish capillary electrophoresis-chemiluminescence method and develop rapid dection tecnique based on Ag(III) chemilulminescence syetem for fast, accurate and sensitive dection of mycotoxins in food. The aims of the present project are:(1) to investigate reaction mechanism of Ag(III) compelx with mycotoxins in luminol solution, acid medium and alkaline medium respectively；(2) to establish much more sensitive capillary electrophoresis-chemiluminescence method for the detection of fumonicin, aflatoxin and so on in food；(3) to develope a rapid dection tecnique of mycotoxin base on Immunoaffinity column-chemiluminescence.

英文关键词： Mycotoxin;Capillary electrophoresis-Chemiluminescence;bis(hydrogenperiodato)argentate(III) complex;Mechanism Investigation