野生和圈养川金丝猴肠道菌群季节动态及功能研究

项目名称： 野生和圈养川金丝猴肠道菌群季节动态及功能研究

项目编号： No.31672318

项目类型： 面上项目

立项/批准年度： 2017

项目学科： 动物学

项目作者： 曾东

作者单位： 四川农业大学

项目金额： 25万元

中文摘要： 川金丝猴是植食性动物，随季节的变化在栖息地中作垂直迁徙。环境可影响宿主肠道菌群的结构和功能，进而影响宿主的健康。本课题组以往的研究表明圈养川金丝猴肠道中栖息着大量而丰富的菌群，且菌群组成及多样性与金丝猴的健康紧密相关。但少见报道野生川金丝猴肠道菌群，特别是未见报道季节变化对野生和圈养川金丝猴肠道菌群及功能的影响，可能是源于研究对象以及研究方法的局限。本项目以不同季节野生和圈养川金丝猴肠道菌群为研究对象，首先应用变性梯度凝胶电泳技术和实时荧光定量PCR技术分析菌群组成及多样性；其次，利用高通量测序技术大规模分析川金丝猴肠道菌群，初步分析关键菌群；最后，应用宏基因组学和代谢组学技术联合分析肠道关键菌群与关键代谢物相关机制，挖掘川金丝猴肠道关键菌群与代谢物，为保护和管理川金丝猴种群提供重要的基础资料，特别是为圈养川金丝猴的日粮组成提供现实指导。

中文关键词： 川金丝猴；肠道菌群；宏基因组学；代谢组学；季节

英文摘要： Golden snub-nosed monkey is a herbivorous animal, has vertical migration in its habitat with the season changes. Gut microbiota assists in intestinal homeostasis and other aspects of the host, including intestinal immune response, digestion, and physiology. A remarkable microbiota was obtained from the feces of golden snub-nosed monkey. In addition, the composition and diversity were closely related to the health of host. However, the research of gut microbiota in wild golden snub-nosed monkey is rare, particularly in the influence of seasonal variation. This could be due to the limitations of the animal studied and research methods. This study aimed to investigate the bacterial communities in the feces of wild and captive golden snub-nosed monkey in different seasons. First, we undertake an analysis of DGGE and real-time PCR to study the composition and diversity of gut microbiota. Second, we used a high-throughput sequencing technology to analyze the intestinal flora. This could help us to obtain a preliminary analysis of the gut microbiota. Finally, we utilize a combination of metagenomics and metabolomics to investigate the mechanism between gut microbiota and metabolites. Our findings provide important basic information for the conservation and management of golden snub-nosed monkey, particularly in the practical guidance of the diet composition in captivity monkey.

英文关键词： Golden snub-nosed monkey;gut microbiota;metagenomics;metabonomics;season