3-D生物芯片中癌细胞侵袭组织的集体行为和动力学研究

项目名称： 3-D生物芯片中癌细胞侵袭组织的集体行为和动力学研究

项目编号： No.11474345

项目类型： 面上项目

立项/批准年度： 2015

项目学科： 数理科学和化学

项目作者： 刘雳宇

作者单位： 重庆大学

项目金额： 105万元

中文摘要： 癌症致命性的根本原因之一是癌细胞侵袭破坏组织，引发癌症转移。先前的研究证实在侵袭过程中癌细胞并不是独立进行侵袭，而是形成群体并表现出多种集体侵袭行为。为了定量深入理解这些复杂行为，我们拟定设计和建立微流体芯片在体外模拟细胞侵袭的三维环境，实验结合理论观测细胞侵袭的集体行为和研究癌细胞不同侵袭行为的内在机制、环境调控以及对组织的影响。并且通过对不同种类细胞群在组织中的动力学研究，理解细胞群力对于细胞转移能力的影响。最后我们将根据实际病例，利用新型3-D打印技术结合图像处理方法构建可模拟人体内部复杂细胞侵袭环境的新型生物芯片，探索其取代现有软刻蚀芯片技术应用于研究癌症等生物问题的可行性。

中文关键词： 软物质；癌症生物物理；细胞力学；集体行为；微流体

英文摘要： One of the fundamental reasons why cancer kills is that cancer cell invasion destructs tissues and causes metastasis. Previous research has shown that cancer cells do not invade independently. Instead they invade in groups and exhibit various collective behaviors. To deepen the understandings of these complex behaviors, we propose to construct new microfluidic chips to mimic cell 3-D invasion microenvironment. The experiments combined with theories will focus on capturing cell colletive invasive behaviors and discovering the mechanism, environmental controls as well as impact on tissues underlying the behaviors. Furthermore we will focus on analyzing group forces of different cellines in extracellular matrix as to clssify their contributions to metastatic potentials. By introducing 3-D printing technology, we will effort on constructing more complex in vitro models to simulate cancer cell invasion microenvironment from clinic examples. The research will explore the possibilities of replacing current soft-lithography techniques on 2-D biochips and future applications in cancer research and other fields of biological studies.

英文关键词： soft matter;cancer biophysics;cell mechanics;collective behavior;microfluidics